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Invitrogen
Phospho-HSF1 (Ser303) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-HSF1 (Ser303) Antibody (PA5-37598) in IHC (P)
Product Details
PA5-37598
Species Reactivity
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Conjugate
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Product Specific Information
A suggested positive control for Western blot is MCF-7 cells; suggested positive control for IHC is human breast carcinoma.
Target Information
All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF1 and HSF2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF1 is necessary for maximal transcription of heat shock genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Bioinformatics
Protein Aliases: Heat shock factor protein 1; Heat shock transcription factor 1; HSF 1; HSTF 1
Gene Aliases: HSF1; HSTF1
UniProt ID: (Human) Q00613
Entrez Gene ID: (Human) 3297
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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