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  • eIF4B Antibodies

Invitrogen

Phospho-EIF4B (Ser504) Polyclonal Antibody

View all (28) eIF4B antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Phospho-EIF4B (Ser504) Polyclonal Antibody

  • Antibody Testing Data (9)
Phospho-EIF4B (Ser504) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-EIF4B (Ser504) Antibody in Immunocytochemistry (ICC/IF)
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Phospho-EIF4B (Ser504) Antibody (PA5-114571) in ICC/IF

Immunocytochemistry analysis of Phospho-EIF4B (Ser504) in HeLa cells (heat shock treatment). Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-114571). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conju... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-EIF4B (Ser504) Antibody in Immunocytochemistry (ICC/IF)
Phospho-EIF4B (Ser504) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EIF4B (Ser504) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EIF4B (Ser504) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EIF4B (Ser504) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EIF4B (Ser504) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EIF4B (Ser504) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EIF4B (Ser504) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-EIF4B (Ser504) Antibody in Western Blot (WB)
Phospho-EIF4B (Ser504) Polyclonal Antibody

Product Details

PA5-114571

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human eIF4B around the phosphorylation site of Ser504.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2899207

Product Specific Information

Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser504.

Target Information

The eukaryotic translation initiation factor 4B (eIF4B) plays a critical role in recruiting the 40S ribosomal subunit to the mRNA. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-4B; Eukaryotic translation initiation factor 4B; hCG_2014609

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Gene Aliases: 2310046H11Rik; AL024095; C85189; EIF-4B; Eif4a2; EIF4B; PRO1843

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UniProt ID: (Human) P23588, (Mouse) Q8BGD9

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Entrez Gene ID: (Human) 1975, (Rat) 300253, (Mouse) 75705

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Function(s)
nucleotide binding RNA binding translation initiation factor activity helicase activity protein binding RNA strand annealing activity RNA strand-exchange activity ribosomal small subunit binding poly(A) RNA binding nucleic acid binding
Process(es)
translational initiation nuclear-transcribed mRNA poly(A) tail shortening formation of translation preinitiation complex cytoplasmic translation regulation of translational initiation eukaryotic translation initiation factor 4F complex assembly translation
It has to be done as per old AB suggested Products section.
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