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  • CDK2 Antibodies

Invitrogen

Phospho-CDK2 (Tyr15) Recombinant Rabbit Monoclonal Antibody (2C4)

1 Published Figure
1 Reference
View all (67) CDK2 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Phospho-CDK2 (Tyr15) Recombinant Rabbit Monoclonal Antibody (2C4)

  • Antibody Testing Data (2)
  • Published Figures (1)
Phospho-CDK2 (Tyr15) Antibody in Western Blot (WB)
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Phospho-CDK2 (Tyr15) Antibody in Western Blot (WB)
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FIGURE: 1 / 3

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Phospho-CDK2 (Tyr15) Antibody (MA5-33128) in WB

Western Blot analysis of Phospho-CDK2 (Tyr15) using a Phospho-CDK2 (Tyr15) Monoclonal antibody (Product # MA5-33128) at a concentration of 0.8 µg/mL. Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate(treated with Pervanadate or not). All lanes: Phospho-CDK2 antibody at 0.8µg/ml. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 34... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-CDK2 (Tyr15) Antibody in Western Blot (WB)
Phospho-CDK2 (Tyr15) Antibody in Western Blot (WB)
Phospho-CDK2 (Tyr15) Antibody in Western Blot (WB)
Phospho-CDK2 (Tyr15) Recombinant Rabbit Monoclonal Antibody (2C4)

Product Details

MA5-33128

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:5,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

ELISA (ELISA)

Assay-Dependent
-

Immunoprecipitation (IP)

1:200-1:1,000
-
Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

2C4

Immunogen

A synthesized peptide derived from human Phospho-CDK2 (Y15)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.6 mg/mL

Purification

Protein G, Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2811944

Target Information

CDK2/cyclinO is a member of the cyclin dependent protein kinase family complexed to CyclinO. CyclinO was used as a specific protein isoform name for the UNG gene which is localized exclusively in the nucleus. In addition, CyclinO was reported to shows significant uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during G1 phase and the protein is turned over during the course of cell cycle. The levels of cyclin A-cdk2 is maximal at the G1/S transition and both cdk2 and cyclin A are found associated with DNA in the initiation complex during replication. Rb protein acts as substrate for cdk2-cyclin E and/or cdk2-cyclin A in vivo. Cdk2 is activated and specifically localized to the nucleus during late G1-, S-, and G2-phase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cdc2-related protein kinase; CDC28; CDC2A; CDK1; Cell division protein kinase 2; Cyclin-dependent kinase 2; EC 2.7.11.22; EC 2.7.11.23; kinase Cdc2; MPF; p33 protein kinase; p33CDK2; p34 protein kinase

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Gene Aliases: CDK2; CDKN2; p33(CDK2)

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UniProt ID: (Human) P24941

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Entrez Gene ID: (Human) 1017

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Function(s)
protein serine/threonine kinase activity cyclin-dependent protein serine/threonine kinase activity protein binding ATP binding kinase activity cyclin binding protein complex binding histone kinase activity metal ion binding
Process(es)
G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle lens development in camera-type eye DNA replication DNA repair potassium ion transport DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest mitotic nuclear division centriole replication meiotic nuclear division Ras protein signal transduction positive regulation of cell proliferation response to toxic substance histone phosphorylation peptidyl-serine phosphorylation organ regeneration mitotic G1 DNA damage checkpoint positive regulation of DNA-dependent DNA replication initiation response to estradiol cellular response to insulin stimulus response to drug response to ethanol positive regulation of transcription, DNA-templated response to cadmium ion centrosome duplication cell division negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle response to cAMP response to electrical stimulus regulation of gene silencing cellular response to nitric oxide regulation of signal transduction by p53 class mediator
It has to be done as per old AB suggested Products section.
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