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Phospho-Bcl-2Thr129 (Thr129) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Phospho-Bcl-2Thr129 (Thr129) Polyclonal Antibody

  • Antibody Testing Data (4)
Phospho-Bcl-2Thr129 (Thr129) Antibody in ELISA (ELISA)
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Phospho-Bcl-2Thr129 (Thr129) Antibody in ELISA (ELISA)
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Phospho-Bcl-2Thr129 (Thr129) Antibody (BS-5220R) in ELISA

Antigen: bs-5220P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1:5000; TMB staining; Read the data in Microplate Reader by 450nm. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-Bcl-2Thr129 (Thr129) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-Bcl-2Thr129 (Thr129) Antibody in Western Blot (WB)
Phospho-Bcl-2Thr129 (Thr129) Antibody in Western Blot (WB)
Phospho-Bcl-2Thr129 (Thr129) Antibody in ELISA (ELISA)
Phospho-Bcl-2Thr129 (Thr129) Polyclonal Antibody

Product Details

BS-5220R

Applications
Tested Dilution
Publications

Western Blot (WB)

1:300
-

Immunohistochemistry (Paraffin) (IHC (P))

1:400
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

Flow Cytometry (Flow)

1:20-1:100
-

ELISA (ELISA)

1:5,000-1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic phosphopeptide derived from rat Bcl-2 around the phosphorylation site of Thr129.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol

Contains

0.02% ProClin 300

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apoptosis regulator Bcl-2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; Bcl2-like protein; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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Gene Aliases: AW986256; Bcl-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50

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UniProt ID: (Human) P10415, (Rat) P49950, (Mouse) P10417

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Entrez Gene ID: (Human) 596, (Rat) 24224, (Mouse) 12043

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Function(s)
protease binding protein binding transcription factor binding channel activity channel inhibitor activity ubiquitin protein ligase binding identical protein binding protein homodimerization activity sequence-specific DNA binding protein heterodimerization activity BH3 domain binding protein phosphatase 2A binding protein phosphatase binding BH domain binding
Process(es)
protein polyubiquitination cell morphogenesis ossification ovarian follicle development metanephros development ureteric bud development branching involved in ureteric bud morphogenesis behavioral fear response response to hypoxia leukocyte homeostasis B cell homeostasis kidney development release of cytochrome c from mitochondria regulation of cell-matrix adhesion lymphocyte homeostasis lymphoid progenitor cell differentiation B cell lineage commitment T cell lineage commitment immune system development response to ischemia renal system process protein dephosphorylation melanin metabolic process regulation of nitrogen utilization cellular calcium ion homeostasis apoptotic process cellular response to DNA damage stimulus response to oxidative stress actin filament organization axonogenesis brain development aging cell aging response to nutrient cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation male gonad development extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage intrinsic apoptotic signaling pathway in response to oxidative stress apoptotic mitochondrial changes response to heat response to toxic substance post-embryonic development organ morphogenesis response to organic substance response to inorganic substance response to iron ion response to aluminum ion response to UV-B response to gamma radiation regulation of gene expression negative regulation of calcium ion transport into cytosol regulation of glycoprotein biosynthetic process mesenchymal cell development positive regulation of neuron maturation response to organic cyclic compound positive regulation of smooth muscle cell migration cell growth single organismal cell-cell adhesion peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cochlear nucleus development gland morphogenesis regulation of transmembrane transporter activity hemopoiesis B cell differentiation T cell differentiation negative regulation of ossification positive regulation of cell growth negative regulation of cell growth melanocyte differentiation negative regulation of cell migration positive regulation of B cell proliferation response to caffeine hair follicle morphogenesis axon regeneration regulation of protein stability endoplasmic reticulum calcium ion homeostasis glomerulus development negative regulation of cellular pH reduction response to insulin regulation of protein localization negative regulation of myeloid cell apoptotic process T cell differentiation in thymus positive regulation of peptidyl-serine phosphorylation response to L-ascorbic acid negative regulation of osteoblast proliferation response to cytokine glial cell apoptotic process response to nicotine organ growth growth positive regulation of multicellular organism growth B cell proliferation cellular response to glucose starvation response to drug response to hydrogen peroxide regulation of apoptotic process T cell homeostasis negative regulation of apoptotic process regulation of programmed cell death positive regulation of catalytic activity CD8-positive, alpha-beta T cell lineage commitment response to peptide hormone pigmentation regulation of protein homodimerization activity regulation of protein heterodimerization activity negative regulation of neuron apoptotic process ear development response to estrogen regulation of viral genome replication response to ethanol positive regulation of melanocyte differentiation negative regulation of mitotic cell cycle negative regulation of retinal cell programmed cell death response to copper ion regulation of mitochondrial membrane permeability focal adhesion assembly developmental pigmentation regulation of developmental pigmentation positive regulation of developmental pigmentation spleen development thymus development response to steroid hormone digestive tract morphogenesis developmental growth oocyte development oligodendrocyte differentiation positive regulation of skeletal muscle fiber development pigment granule organization homeostasis of number of cells within a tissue regulation of catalytic activity B cell receptor signaling pathway response to glucocorticoid neuron apoptotic process response to corticosterone response to cAMP response to folic acid response to electrical stimulus defense response to virus regulation of cell cycle regulation of mitochondrial membrane potential regulation of calcium ion transport transmembrane transport intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to organic substance cellular response to hypoxia reactive oxygen species metabolic process extrinsic apoptotic signaling pathway in absence of ligand negative regulation of G1/S transition of mitotic cell cycle negative regulation of anoikis negative regulation of apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway humoral immune response female pregnancy response to radiation negative regulation of autophagy negative regulation of mitochondrial depolarization positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway negative regulation of reactive oxygen species metabolic process positive regulation of intrinsic apoptotic signaling pathway response to acid chemical
It has to be done as per old AB suggested Products section.
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