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  • Primary Antibodies ›
  • Annexin A4 Antibodies

Invitrogen

Phospho-Annexin A4 (Tyr255) Polyclonal Antibody

View all (23) Annexin A4 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Phospho-Annexin A4 (Tyr255) Polyclonal Antibody

  • Antibody Testing Data (3)
Phospho-Annexin A4 (Tyr255) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Phospho-Annexin A4 (Tyr255) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Phospho-Annexin A4 (Tyr255) Antibody (PA5-105361) in IHC (P)

Immunohistochemistry analysis of paraffin-embedded Phospho-Annexin A4 (Tyr255) in human kidney tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Annexin A4 (Tyr255) polyclonal antibody (Product # PA5-105361) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-Annexin A4 (Tyr255) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-Annexin A4 (Tyr255) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Phospho-Annexin A4 (Tyr255) Antibody in Western Blot (WB)
Phospho-Annexin A4 (Tyr255) Polyclonal Antibody

Product Details

PA5-105361

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ANXA4(Accession P09525), corresponding to amino acid residues around phosphorylated Tyr255.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2816789

Product Specific Information

Antibody detects endogenous levels of Annexin IV only when phosphorylated at Tyr255.

Target Information

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 35-beta calcimedin; Annexin A4; Annexin IV; annexin IV (placental anticoagulant protein II); Annexin-4; ANXA 4; Carbohydrate-binding protein p33/p41; Chromobindin-4; Endonexin I; epididymis secretory protein Li 274; Lipocortin IV; P32.5; PAP-II; Placental anticoagulant protein II; PP4-X; proliferation-inducing gene 28; proliferation-inducing protein 28; Protein II

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Gene Aliases: AI265406; AIV; ANX4; ANXA4; AW106930; HEL-S-274; P32.5; PAP-II; PIG28; PP4-X; Xanx-4; ZAP36

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UniProt ID: (Human) P09525, (Mouse) P97429

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Entrez Gene ID: (Human) 307, (Mouse) 11746

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Function(s)
phospholipase inhibitor activity calcium ion binding protein binding calcium-dependent phospholipid binding identical protein binding calcium-dependent protein binding NF-kappaB binding
Process(es)
kidney development regulation of transcription from RNA polymerase II promoter Notch signaling pathway negative regulation of NF-kappaB transcription factor activity negative regulation of interleukin-8 secretion signal transduction epithelial cell differentiation negative regulation of apoptotic process negative regulation of catalytic activity
It has to be done as per old AB suggested Products section.
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