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Invitrogen

Phospho-AKT1 (Ser124) Recombinant Rabbit Monoclonal Antibody (1O8H2)

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Datasheet
Protocols
Questions & Answers

Cite Phospho-AKT1 (Ser124) Recombinant Rabbit Monoclonal Antibody (1O8H2)

  • Antibody Testing Data (1)
Phospho-AKT1 (Ser124) Antibody in Western Blot (WB)
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Phospho-AKT1 (Ser124) Antibody in Western Blot (WB)
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Phospho-AKT1 (Ser124) Antibody (MA5-35842) in WB

Western blot analysis of Phospho-AKT1 (Ser124) in extracts of MCF7 cells. Samples were incubated with Phospho-AKT1 (Ser124) Monoclonal antibody (Product # MA5-35842) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. MCF7 cells were treated by CIP (20 µL/400 µL) at 37°C for 1 hour. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% BSA. D... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Phospho-AKT1 (Ser124) Antibody in Western Blot (WB)
Phospho-AKT1 (Ser124) Recombinant Rabbit Monoclonal Antibody (1O8H2)

Product Details

MA5-35842

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1O8H2

Immunogen

A synthetic phosphorylated peptide around S124 of human Akt1 (P31749)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.43 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849741

Product Specific Information

Immunogen sequence: SGSPS

Target Information

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AKT1m; C-AKT; CAKT; PKB; PKB alpha; PKB beta; PKB gamma; PKBG; Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; rac protein kinase alpha; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1

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Gene Aliases: AKT; AKT1; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA

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UniProt ID: (Human) P31749

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Entrez Gene ID: (Human) 207

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein kinase C binding protein binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding nitric-oxide synthase regulator activity GTPase activating protein binding identical protein binding phosphatidylinositol-3,4-bisphosphate binding protein phosphatase 2A binding 14-3-3 protein binding
Process(es)
protein import into nucleus, translocation osteoblast differentiation maternal placenta development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation glycogen biosynthetic process regulation of glycogen biosynthetic process glucose metabolic process translation regulation of translation cellular protein modification process protein phosphorylation negative regulation of protein kinase activity nitric oxide biosynthetic process activation-induced cell death of T cells inflammatory response cellular response to DNA damage stimulus response to oxidative stress signal transduction G-protein coupled receptor signaling pathway germ cell development aging cell proliferation insulin receptor signaling pathway apoptotic mitochondrial changes response to heat negative regulation of autophagy negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of glucose metabolic process negative regulation of endopeptidase activity regulation of neuron projection development regulation of phosphatidylinositol 3-kinase signaling glucose transport phosphorylation protein ubiquitination peptidyl-serine phosphorylation peptidyl-threonine phosphorylation spinal cord development cell projection organization cell differentiation protein catabolic process platelet activation hyaluronan metabolic process positive regulation of cell growth regulation of cell migration endocrine pancreas development T cell costimulation regulation of myelination positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle lipopolysaccharide-mediated signaling pathway negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity response to food positive regulation of cellular protein metabolic process peripheral nervous system myelin maintenance positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation response to fluid shear stress intracellular signal transduction cellular response to vascular endothelial growth factor stimulus ERBB2 signaling pathway glucose homeostasis positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process positive regulation of epidermal growth factor receptor signaling pathway negative regulation of cell size negative regulation of proteolysis positive regulation of vasoconstriction positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import negative regulation of JNK cascade protein autophosphorylation positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway phosphatidylinositol-mediated signaling regulation of nitric-oxide synthase activity positive regulation of nitric-oxide synthase activity positive regulation of sequence-specific DNA binding transcription factor activity striated muscle cell differentiation response to growth hormone mammary gland epithelial cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development response to UV-A cellular response to mechanical stimulus cellular response to epidermal growth factor stimulus cellular response to prostaglandin E stimulus cellular response to organic cyclic compound cellular response to hypoxia establishment of protein localization to mitochondrion maintenance of protein location in mitochondrion positive regulation of establishment of protein localization to plasma membrane negative regulation of release of cytochrome c from mitochondria cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis chemical synaptic transmission, postsynaptic negative regulation of protein kinase activity by protein phosphorylation positive regulation of protein localization to nucleus negative regulation of neuron death regulation of signal transduction by p53 class mediator regulation of cell cycle checkpoint negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
It has to be done as per old AB suggested Products section.
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