Rockland

Pax7 Polyclonal Antibody

Name: Pax7 Polyclonal Antibody
Brand: Rockland
SKU: 600-401-GR7

View all (34) PAX7 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

FIGURE: 1 / 1

Pax7 Antibody (600-401-GR7) in WB

Western Blot of Rabbit anti-Pax7 antibody. Lane 1: Opal Pre-stained ladder (p/n MB-210-0500). Lane 2: Mouse muscle protein lysate. Lane 3: C2C12 Lysate (p/n W09-001-GL7). Lane 4: Ms spleen lysate (p/n W10-000-T029). Lane 5: Ms brain lysate (p/n W10-000-T004). Lane 6: HEK293T Lysate (W09-001-GX5). Lane 7: HeLa WCL (p/n W09-000-364). Lane 8: MCF7 Lysate (p/n W09-000-360). Lane 9: Jurkat Lysate (p/n W09-001-370). Lane 10: A431 Lysate (p/n W09-000-361). Lane 11: A549 Lysate (p/n W09-001-372). Lane 12: LNCap Lysate (p/n W09-001-GJ9). Lane 13... View More

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Product Details

600-401-GR7

Applications

Tested Dilution

Publications

Western Blot (WB)

1.0 µg/mL

ELISA (ELISA)

1:10,000-1:40,000

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Pax7 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Pax7 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

A BLAST analysis was used to suggest cross reactivity with Pax7 protein from mouse (100% homology) and human (66.7% homology) for the immunizing sequence. Cross-reactivity with Pax7 from other sources has not been determined.

Target Information

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ37460; paired box gene 7; Paired box protein Pax-7; paired box transcription factor PAX7; PAX7A

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Gene Aliases: Pax-7; Pax7

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UniProt ID: (Mouse) P47239

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Entrez Gene ID: (Mouse) 18509

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Function(s)

transcription factor activity, RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding

Process(es)

chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development muscle organ development skeletal muscle tissue development organ morphogenesis regulation of cell fate commitment regulation of gene expression skeletal muscle satellite cell commitment spinal cord association neuron differentiation dorsal/ventral neural tube patterning positive regulation of histone methylation regulation of protein binding skeletal muscle tissue regeneration positive regulation of transcription from RNA polymerase II promoter neuron fate commitment embryonic skeletal system development cartilage development muscle tissue morphogenesis positive regulation of myoblast proliferation

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Pax7 Polyclonal Antibody

Pax7 Antibody (600-401-GR7) in WB

Product Details

600-401-GR7

Western Blot (WB)

ELISA (ELISA)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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