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  • Pancreatic Amylase Antibodies

Invitrogen

Pancreatic Amylase Polyclonal Antibody

View all (19) Pancreatic Amylase antibodies

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Cite Pancreatic Amylase Polyclonal Antibody

  • Antibody Testing Data (1)
Pancreatic Amylase Antibody in Western Blot (WB)
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Pancreatic Amylase Antibody in Western Blot (WB)
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Pancreatic Amylase Antibody (PA5-117115) in WB

Western blot analysis of Pancreatic Amylase in extracts of various cell lines. Samples were incubated with Pancreatic Amylase Polyclonal antibody (Product # PA5-117115) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Pancreatic Amylase Antibody in Western Blot (WB)
Pancreatic Amylase Polyclonal Antibody

Product Details

PA5-117115

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 16-511 of human AMY2A (NP_0006901)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.13 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2901745

Product Specific Information

Positive Samples: Mouse intestine, Rat intestine

Immunogen sequence: QYSPNTQQGR TSIVHLFEWR WVDIALECER YLAPKGFGGV QVSPPNENVA IYNPFRPWWE RYQPVSYKLC TRSGNEDEFR NMVTRCNNVG VRIYVDAVIN HMCGNAVSAG TSSTCGSYFN PGSRDFPAVP YSGWDFNDGK CKTGSGDIEN YNDATQVRDC RLTGLLDLAL EKDYVRSKIA EYMNHLIDIG VAGFRLDASK HMWPGDIKAI LDKLHNLNSN WFPAGSKPFI YQEVIDLGGE PIKSSDYFGN GRVTEFKYGA KLGTVIRKWN GEKMSYLKNW GEGWGFVPSD RALVFVDNHD NQRGHGAGGA SILTFWDARL YKMAVGFMLA HPYGFTRVMS SYRWPRQFQN GNDVNDWVGP PNNNGVIKEV TINPDTTCGN DWVCEHRWRQ IRNMVIFRNV VDGQPFTNWY DNGSNQVAFG RGNRGFIVFN NDDWSFSLTL QTGLPAGTYC DVISGDKING NCTGIKIYVS DDGKAHFSIS NSAEDPFIAI HAESKL

Target Information

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch andglycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 1,4-alpha-D-glucan glucanohydrolase; 1,4-alpha-D-glucan glucanohydrolase 2B; alpha-amylase; Alpha-amylase 2B; amylase; amylase 2-2, pancreatic; amylase 2a4; amylase 2a4, pancreatic; amylase 2b; amylase 2b, pancreatic; Carcinoid alpha-amylase; glycogenase; PA; Pancreatic alpha-amylase; Pancreatic alpha-amylase 2a5; pancreatic amylase alpha 2A

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Gene Aliases: Amy-X; AMY2; Amy2-1; Amy2-2; AMY2A; Amy2a5; AMY2B; AMY3; ENSMUSG00000074267; HXA; mAmy2-1; mAmy2-3; mAmy2-6; OTTMUSG00000022432; OTTMUSG00000022433; OTTMUSG00000022462; PA

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UniProt ID: (Human) P04746, (Human) P19961, (Mouse) P00688

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Entrez Gene ID: (Human) 279, (Human) 280, (Mouse) 100043207, (Mouse) 100043684, (Mouse) 545562

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Function(s)
alpha-amylase activity calcium ion binding chloride ion binding metal ion binding
Process(es)
carbohydrate metabolic process carbohydrate catabolic process polysaccharide digestion digestion biological_process
It has to be done as per old AB suggested Products section.
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