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  • Primary Antibodies ›
  • PRP8 Antibodies

Bethyl Laboratories

PRPF8 Polyclonal Antibody

View all (12) PRP8 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite PRPF8 Polyclonal Antibody

  • Antibody Testing Data (3)
PRPF8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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PRPF8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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PRPF8 Antibody (A303-921A) in IHC (P)

Detection of human PRPF8 by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-PRPF8 (Product # A303-920A) (Lot 1, left panel), (Product # A303-921A) (Lot 1, middle panel) and (Product # A303-922A) (Lot 1, right panel) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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PRPF8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
PRPF8 Antibody in Western Blot (WB)
PRPF8 Antibody in Immunoprecipitation (IP)
PRPF8 Polyclonal Antibody

Product Details

A303-921A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunohistochemistry (IHC)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000
-

Immunoprecipitation (IP)

2-10 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 1825 and 1875 of Human Pre-mRNA-Processing Factor 8.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 220 kDa U5 snRNP-specific protein; apoptosis-regulated protein 1; apoptosis-regulated protein 2; H PRPC8; p220; Pre-mRNA-processing-splicing factor 8; precursor mRNA processing protein; PRP8 homolog; PRP8 pre-mRNA processing factor 8 homolog; spl; Splicing factor Prp8; U5 snRNP-specific protein (220 kD), ortholog of S. cerevisiae Prp8p

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Gene Aliases: AU019467; D11Bwg0410e; DBF3/PRP8; HPRP8; PRP8; PRPC8; PRPF8; RP13; Sfprp8l; SNRNP220

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UniProt ID: (Human) Q6P2Q9, (Mouse) Q99PV0

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Entrez Gene ID: (Human) 10594, (Mouse) 192159

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Function(s)
second spliceosomal transesterification activity protein binding U6 snRNA binding U1 snRNA binding U2 snRNA binding U5 snRNA binding poly(A) RNA binding K63-linked polyubiquitin binding pre-mRNA intronic binding nucleic acid binding RNA binding
Process(es)
spliceosomal tri-snRNP complex assembly RNA splicing, via transesterification reactions mRNA splicing, via spliceosome mRNA processing RNA splicing cellular response to lipopolysaccharide cellular response to tumor necrosis factor
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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