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  • Primary Antibodies ›
  • PP2C beta Antibodies

Invitrogen

PP2C beta Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite PP2C beta Polyclonal Antibody

  • Antibody Testing Data (2)
PP2C beta Antibody in Immunocytochemistry (ICC/IF)
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PP2C beta Antibody in Immunocytochemistry (ICC/IF)
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PP2C beta Antibody (PA5-115784) in ICC/IF

Immunocytochemistry analysis of PP2A alpha in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115784) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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PP2C beta Antibody in Immunocytochemistry (ICC/IF)
PP2C beta Antibody in Western Blot (WB)
PP2C beta Polyclonal Antibody

Product Details

PA5-115784

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human PPM1B(Accession O75688), corresponding to amino acid residues E412-E462.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2900419

Product Specific Information

Antibody detects endogenous levels of total PP2C alpha.

Target Information

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: PP2C-beta; Protein phosphatase 1B; protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform; Protein phosphatase 2C isoform beta; protein phosphatase 2C-like protein; protein phosphatase, Mg2+/Mn2+ dependent, 1B; Ser/Thr protein phosphatase type 2C beta 2 isoform

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Gene Aliases: PP2C-beta; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX; PPM1B

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UniProt ID: (Human) O75688

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Entrez Gene ID: (Human) 5495

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Function(s)
magnesium ion binding protein serine/threonine phosphatase activity protein binding manganese ion binding
Process(es)
protein dephosphorylation N-terminal protein myristoylation negative regulation of interferon-beta production peptidyl-threonine dephosphorylation negative regulation of NF-kappaB import into nucleus negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of defense response to virus
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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