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  • Primary Antibodies ›
  • POLR2E Antibodies

Invitrogen

POLR2E Monoclonal Antibody (OTI3B5)

1 Published Figure
1 Reference
View all (16) POLR2E antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite POLR2E Monoclonal Antibody (OTI3B5)

  • Antibody Testing Data (11)
  • Published Figures (1)
POLR2E Antibody in Immunocytochemistry (ICC/IF)
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POLR2E Antibody in Immunocytochemistry (ICC/IF)
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FIGURE: 1 / 12

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POLR2E Antibody (MA5-25454) in ICC/IF

Immunofluorescent analysis of POLR2E in COS7 cells. Cells were transfected with a plasmid overexpressing POLR2E and probed with a POLR2E monoclonal antibody (Product # MA5-25454). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLR2E Antibody in Immunocytochemistry (ICC/IF)
POLR2E Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2E Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2E Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2E Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2E Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2E Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2E Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2E Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2E Antibody in Western Blot (WB)
POLR2E Antibody in Western Blot (WB)
POLR2E Antibody in Western Blot (WB)
POLR2E Monoclonal Antibody (OTI3B5)

Product Details

MA5-25454

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:150
-

Immunocytochemistry (ICC/IF)

1:50-1:100
-

Flow Cytometry (Flow)

1:100
-
Product Specifications

Species Reactivity

Dog, Human, Mouse, Non-human primate, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI3B5

Immunogen

Full length human recombinant protein of POLR2E produced in HEK293T cell
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.9 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2725060

Target Information

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerase subunit RPABC1; DNA-directed RNA polymerases I, II, and III subunit RPABC1; polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; polymerase (RNA) II subunit E; RNA polymerases I, II, and III subunit ABC1; RPB5 homolog; XAP4

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Gene Aliases: 2410021N14Rik; 25kDa; AW208866; hRPB25; hsRPB5; POLR2E; RPABC1; RPB5; XAP4

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UniProt ID: (Human) P19388, (Mouse) Q80UW8, (Rat) B0BNE2

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Entrez Gene ID: (Dog) 611859, (Human) 5434, (Mouse) 66420, (Rat) 690966

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Function(s)
RNA polymerase I activity RNA polymerase II activity RNA polymerase III activity DNA binding DNA-directed RNA polymerase activity protein binding molecular_function
Process(es)
transcription, DNA-templated transcription from RNA polymerase II promoter mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase I promoter termination of RNA polymerase I transcription transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping transcription from RNA polymerase III promoter fibroblast growth factor receptor signaling pathway gene expression viral process gene silencing by RNA positive regulation of type I interferon production somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of gene expression, epigenetic positive regulation of viral transcription transcription from RNA polymerase I promoter biological_process
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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