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  • POLR2D Antibodies

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POLR2D Monoclonal Antibody (1E4-A5)

View all (8) POLR2D antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite POLR2D Monoclonal Antibody (1E4-A5)

  • Antibody Testing Data (1)
POLR2D Antibody in Western Blot (WB)
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POLR2D Antibody in Western Blot (WB)
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POLR2D Antibody (H00005433-M01) in WB

Western Blot analysis of POLR2D expression in transfected 293T cell line by POLR2D monoclonal antibody (M01), clone 1E4-A5. Lane 1: POLR2D transfected lysate(16.3 KDa). Lane 2: Non-transfected lysate. . {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLR2D Antibody in Western Blot (WB)

Product Details

H00005433-M01

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

ELISA (ELISA)

10 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1E4-A5

Immunogen

POLR2D (AAH17205, 1 a.a. approximately 142 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Product may be used with Western Blot (Transfected lysate).

Immunogen sequence: MAAGGSDPRA GDVEEDASQL IFPKGFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQK KLHKFELACL ANLCPETAEE SKALIPSLEG RFEDEELQQI LDDIQTKRSF QY

Target Information

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA-directed RNA polymerase II 16 kDa polypeptide; DNA-directed RNA polymerase II subunit D; DNA-directed RNA polymerase II subunit RPB4; plasma; polymerase (RNA) II (DNA directed) polypeptide D; polymerase (RNA) II subunit D; RNA polymerase II 16 kDa subunit; RNA polymerase II subunit B4; RNA polymerase II subunit hsRBP4; RPB16

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Gene Aliases: HSRBP4; HSRPB4; POLR2D; RBP4; RPB16

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UniProt ID: (Human) O15514

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Entrez Gene ID: (Human) 5433

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Function(s)
nucleotide binding single-stranded DNA binding single-stranded RNA binding DNA-directed RNA polymerase activity translation initiation factor binding
Process(es)
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA mRNA export from nucleus in response to heat stress recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of translational initiation positive regulation of viral transcription
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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