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  • Primary Antibodies ›
  • POLR2D Antibodies

Invitrogen

POLR2D Polyclonal Antibody

View all (8) POLR2D antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite POLR2D Polyclonal Antibody

  • Antibody Testing Data (3)
POLR2D Antibody in Immunocytochemistry (ICC/IF)
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POLR2D Antibody in Immunocytochemistry (ICC/IF)
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POLR2D Antibody (PA5-95964) in ICC/IF

Immunofluorescence analysis of POLR2D in A549 cells. Samples were incubated with POLR2D Polyclonal antibody (Product # PA5-95964). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLR2D Antibody in Immunocytochemistry (ICC/IF)
POLR2D Antibody in Western Blot (WB)
POLR2D Antibody in Immunoprecipitation (IP)
POLR2D Polyclonal Antibody

Product Details

PA5-95964

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:10-1:100
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human POLR2D (NP_0047961)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.24 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2807766

Product Specific Information

Immunogen sequence: MAAGGSDPRA GDVEEDASQL IFPKEFETAE TLLNSEVHML LEHRKQQNES AEDEQELSEV FMKTLNYTAR FSRFKNRETI ASVRSLLLQK KLHKFELACL ANLCPETAEE SKALIPSLEG RFEDEELQQI LDDIQTKRSF QY; Positive Samples: MCF-7, SW480, SW620, HepG2, SK-HEPC, Mouse spleen; Cellular Location: Nucleus

Target Information

This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA-directed RNA polymerase II 16 kDa polypeptide; DNA-directed RNA polymerase II subunit D; DNA-directed RNA polymerase II subunit RPB4; plasma; polymerase (RNA) II (DNA directed) polypeptide D; polymerase (RNA) II subunit D; RNA polymerase II 16 kDa subunit; RNA polymerase II subunit B4; RNA polymerase II subunit hsRBP4; RPB16

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Gene Aliases: 2310002G05Rik; 2610028L19Rik; HSRBP4; HSRPB4; POLR2D; RBP4; RPB16

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UniProt ID: (Human) O15514, (Mouse) Q9D7M8

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Entrez Gene ID: (Human) 5433, (Mouse) 69241

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Function(s)
nucleotide binding single-stranded DNA binding single-stranded RNA binding DNA-directed RNA polymerase activity translation initiation factor binding catalytic activity
Process(es)
nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter mRNA export from nucleus in response to heat stress recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex cellular metabolic process positive regulation of translational initiation mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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