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  • Primary Antibodies ›
  • POLR2C Antibodies

Invitrogen

POLR2C Polyclonal Antibody

View all (10) POLR2C antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite POLR2C Polyclonal Antibody

  • Antibody Testing Data (6)
POLR2C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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POLR2C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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FIGURE: 1 / 6

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POLR2C Antibody (PA5-102484) in IHC (P)

Immunohistochemistry analysis of POLR2C in mouse brain tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with POLR2C polyclonal antibody (Product # PA5-102484) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLR2C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2C Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2C Antibody in Western Blot (WB)
POLR2C Antibody in Western Blot (WB)
POLR2C Antibody in Western Blot (WB)
POLR2C Polyclonal Antibody

Product Details

PA5-102484

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human POLR2C(Accession P19387), corresponding to amino acid residues H194-I244.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2851886

Product Specific Information

Antibody detects endogenous levels of total POLR2C.

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DNA-directed RNA polymerase II 33 kDa polypeptide; DNA-directed RNA polymerase II subunit C; DNA-directed RNA polymerase II subunit RPB3; polymerase (RNA) II (DNA directed) polypeptide C, 33kDa; polymerase (RNA) II subunit C; polymerase II polypeptide C; RNA polymerase II 3; RNA polymerase II subunit 3; RNA polymerase II subunit B3; RPB31; RPB33

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Gene Aliases: 33kDa; A-152E5.7; hRPB33; hsRPB3; mRBP31; POLR2C; RPB3; RPB31; Rpo2-3

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UniProt ID: (Human) P19387, (Mouse) P97760

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Entrez Gene ID: (Human) 5432, (Rat) 361365, (Mouse) 20021

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Function(s)
RNA polymerase II activity DNA binding protein binding protein dimerization activity DNA-directed RNA polymerase activity
Process(es)
transcription from RNA polymerase II promoter transcription, DNA-templated mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription
It has to be done as per old AB suggested Products section.
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