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  • POLR2B Antibodies

Invitrogen

POLR2B Recombinant Rabbit Monoclonal Antibody (HL1043)

View all (9) POLR2B antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite POLR2B Recombinant Rabbit Monoclonal Antibody (HL1043)

  • Antibody Testing Data (1)
POLR2B Antibody in Western Blot (WB)
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POLR2B Antibody in Western Blot (WB)
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POLR2B Antibody (MA5-46920) in WB

Western blot analysis of POLR2B in 293T whole cell extracts (30 µg) separated by 5% SDS-PAGE. Membrane was blotted with POLR2B Monoclonal antibody (Product # MA5-46920) using a dilution of 1:1,000 followed by HRP-conjugated anti-rabbit IgG secondary antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLR2B Antibody in Western Blot (WB)
POLR2B Recombinant Rabbit Monoclonal Antibody (HL1043)

Product Details

MA5-46920

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-
Product Specifications

Species Reactivity

Dog, Cat, Human, Mouse, Rat, Zebrafish

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

HL1043

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2937992

Product Specific Information

Store as concentrated solution.

Centrifuge briefly prior to opening vial.

Target Information

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3'-5' exoribonuclease; DNA directed RNA polymerase II polypeptide B; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; DNA-directed RNA polymerase II subunit RPB2; hs hRPB140; hsRPB2; polymerase (RNA) II (DNA directed) polypeptide B, 140kDa; polymerase (RNA) II subunit B; RNA polymerase II second largest subunit; RNA polymerase II subunit 2; RNA polymerase II subunit B2; RNA-directed RNA polymerase II subunit RPB2; rpb 2

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Gene Aliases: hRPB140; POL2RB; POLR2B; Rpb140; RPB2; zgc:109952

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UniProt ID: (Human) P30876, (Mouse) Q8CFI7

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Entrez Gene ID: (Dog) 475152, (Human) 5431, (Rat) 289561, (Mouse) 231329, (Zebrafish) 100149268

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Function(s)
RNA polymerase II activity DNA binding chromatin binding protein binding ribonucleoside binding poly(A) RNA binding metal ion binding molecular_function DNA-directed RNA polymerase activity transferase activity nucleotidyltransferase activity
Process(es)
transcription, DNA-templated transcription from RNA polymerase II promoter biological_process mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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