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  • Primary Antibodies ›
  • POLR2B Antibodies

Invitrogen

POLR2B Polyclonal Antibody

View all (9) POLR2B antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite POLR2B Polyclonal Antibody

  • Antibody Testing Data (4)
POLR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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POLR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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POLR2B Antibody (PA5-76189) in IHC (P)

Immunohistochemistry analysis of POLR2B in paraffin-embedded mouse liver. Samples were incubated with POLR2B polyclonal antibody (Product # PA5-76189) using a dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2B Antibody in Western Blot (WB)

Product Details

PA5-76189

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein of human POLR2B.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2719916

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Target Information

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3'-5' exoribonuclease; DNA directed RNA polymerase II polypeptide B; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; DNA-directed RNA polymerase II subunit RPB2; hs hRPB140; hsRPB2; polymerase (RNA) II (DNA directed) polypeptide B, 140kDa; polymerase (RNA) II subunit B; RNA polymerase II second largest subunit; RNA polymerase II subunit 2; RNA polymerase II subunit B2; RNA-directed RNA polymerase II subunit RPB2; rpb 2

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Gene Aliases: hRPB140; POL2RB; POLR2B; Rpb140; RPB2

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UniProt ID: (Human) P30876, (Mouse) Q8CFI7

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Entrez Gene ID: (Human) 5431, (Rat) 289561, (Mouse) 231329

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Function(s)
RNA polymerase II activity DNA binding chromatin binding protein binding ribonucleoside binding poly(A) RNA binding metal ion binding molecular_function DNA-directed RNA polymerase activity transferase activity nucleotidyltransferase activity
Process(es)
transcription from RNA polymerase II promoter biological_process transcription, DNA-templated mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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