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  • Primary Antibodies ›
  • POLR2B Antibodies

Invitrogen

POLR2B Polyclonal Antibody

View all (9) POLR2B antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite POLR2B Polyclonal Antibody

  • Antibody Testing Data (2)
POLR2B Antibody in Western Blot (WB)
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POLR2B Antibody in Western Blot (WB)
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POLR2B Antibody (PA5-118173) in WB

Western Blot using POLR2B Polyclonal Antibody (Product # PA5-118173) at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 134 kDa. Observed band size: 130 kDa. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLR2B Antibody in Western Blot (WB)
POLR2B Antibody in Immunoprecipitation (IP)

Product Details

PA5-118173

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunoprecipitation (IP)

1-5 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived Human POLR2B fragment

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography, Protein A

Storage buffer

PBS, pH 7

Contains

0.03% ProClin 300

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2902773

Target Information

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3'-5' exoribonuclease; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; DNA-directed RNA polymerase II subunit RPB2; hs hRPB140; hsRPB2; polymerase (RNA) II (DNA directed) polypeptide B, 140kDa; polymerase (RNA) II subunit B; RNA polymerase II second largest subunit; RNA polymerase II subunit 2; RNA polymerase II subunit B2; RNA-directed RNA polymerase II subunit RPB2; rpb 2

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Gene Aliases: hRPB140; POL2RB; POLR2B; RPB2

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UniProt ID: (Human) P30876

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Entrez Gene ID: (Human) 5431

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Function(s)
RNA polymerase II activity DNA binding chromatin binding protein binding ribonucleoside binding poly(A) RNA binding metal ion binding
Process(es)
mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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