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  • Primary Antibodies ›
  • POLR2B Antibodies

Proteintech

POLR2B Polyclonal Antibody

2 References
View all (9) POLR2B antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite POLR2B Polyclonal Antibody

  • Antibody Testing Data (6)
POLR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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POLR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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POLR2B Antibody (20370-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human brain using 20370-1-AP (POLR2B-Specific antibody) at dilution of 1:400 (under 40x lens). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
POLR2B Antibody in Western Blot (WB)
POLR2B Antibody in Western Blot (WB)
POLR2B Antibody in Immunoprecipitation (IP)
POLR2B Antibody in Immunoprecipitation (IP)

Product Details

20370-1-AP

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200
-

Immunoprecipitation (IP)

0.5-4.0 µg
-

ChIP assay (ChIP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.21 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

The antibody is specific to POLR2B.

Target Information

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 3'-5' exoribonuclease; DNA directed RNA polymerase II polypeptide B; DNA-directed RNA polymerase II 140 kDa polypeptide; DNA-directed RNA polymerase II subunit B; DNA-directed RNA polymerase II subunit RPB2; hs hRPB140; hsRPB2; polymerase (RNA) II (DNA directed) polypeptide B, 140kDa; polymerase (RNA) II subunit B; RNA polymerase II second largest subunit; RNA polymerase II subunit 2; RNA polymerase II subunit B2; RNA-directed RNA polymerase II subunit RPB2; rpb 2

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Gene Aliases: hRPB140; POL2RB; POLR2B; Rpb140; RPB2

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UniProt ID: (Human) P30876, (Mouse) Q8CFI7

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Entrez Gene ID: (Human) 5431, (Rat) 289561, (Mouse) 231329

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Function(s)
RNA polymerase II activity DNA binding chromatin binding protein binding ribonucleoside binding poly(A) RNA binding metal ion binding molecular_function DNA-directed RNA polymerase activity transferase activity nucleotidyltransferase activity
Process(es)
transcription from RNA polymerase II promoter biological_process transcription, DNA-templated mRNA splicing, via spliceosome transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase II promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping fibroblast growth factor receptor signaling pathway gene expression gene silencing by RNA somatic stem cell population maintenance snRNA transcription from RNA polymerase II promoter positive regulation of viral transcription
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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