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  • POLD1 Antibodies

Invitrogen

POLD1 Recombinant Rabbit Monoclonal Antibody (4B6I3)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite POLD1 Recombinant Rabbit Monoclonal Antibody (4B6I3)

  • Antibody Testing Data (1)
POLD1 Antibody in Western Blot (WB)
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POLD1 Antibody in Western Blot (WB)
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POLD1 Antibody (MA5-35464) in WB

Western blot analysis of POLD1 in extracts of various cell lines. Samples were incubated with POLD1 Monoclonal antibody (Product # MA5-35464) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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POLD1 Antibody in Western Blot (WB)
POLD1 Recombinant Rabbit Monoclonal Antibody (4B6I3)

Product Details

MA5-35464

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:6,000
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

4B6I3

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1000-1107 of human POLD1 (P28340)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849365

Product Specific Information

Immunogen sequence: GGLLAFAKRR NCCIGCRTVL SHQGAVCEFC QPRESELYQK EVSHLNALEE RFSRLWTQCQ RCQGSLHEDV ICTSRDCPIF YMRKKVRKDL EDQEQLLRRF GPPGPEAW

Target Information

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3'-5' exodeoxyribonuclease; CDC2 homolog; DNA polymerase delta 1, catalytic domain; DNA polymerase delta catalytic subunit; DNA polymerase delta, catalytic subunit; DNA polymerase subunit delta p125; DNA-directed DNA polymerase delta 1; POL D; polymerase (DNA directed), delta 1, catalytic subunit 125kDa; polymerase (DNA) delta 1, catalytic subunit; Polymerase D

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Gene Aliases: 125kDa; CDC2; CRCS10; MDPL; POLD; POLD1

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UniProt ID: (Human) P28340, (Rat) O54747, (Mouse) P52431

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Entrez Gene ID: (Human) 5424, (Rat) 59294, (Mouse) 18971

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Function(s)
nucleotide binding DNA binding chromatin binding damaged DNA binding DNA-directed DNA polymerase activity protein binding 3'-5'-exodeoxyribonuclease activity enzyme binding metal ion binding 4 iron, 4 sulfur cluster binding 3'-5' exonuclease activity nucleic acid binding nuclease activity exonuclease activity transferase activity nucleotidyltransferase activity hydrolase activity iron-sulfur cluster binding
Process(es)
telomere maintenance via recombination telomere maintenance DNA synthesis involved in DNA repair DNA replication DNA strand elongation involved in DNA replication DNA repair transcription-coupled nucleotide-excision repair base-excision repair, gap-filling nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling mismatch repair response to UV translesion synthesis nucleotide-excision repair, DNA incision cellular response to UV DNA damage response, detection of DNA damage DNA replication proofreading fatty acid homeostasis DNA catabolic process, exonucleolytic DNA-dependent DNA replication DNA biosynthetic process nucleic acid phosphodiester bond hydrolysis
It has to be done as per old AB suggested Products section.
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