Alomone Labs, LTD

PICK1 Polyclonal Antibody

Name: PICK1 Polyclonal Antibody
Brand: Alomone Labs, LTD
SKU: APZ-014-200UL

View all (15) PICK1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)

PICK1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

FIGURE: 1 / 3

PICK1 Antibody (APZ-014-200UL) in IHC (F)

Expression of PICK1 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-PICK1 Antibody (#APZ-014), (1:400), followed by Anti-rabbit-Cy3 Antibody . PICK1 staining (red in the hippocampal CA3 region) is detected in neurons (arrows). Nuclei are stained with DAPI (blue).

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Immunogen image

Immunogen Image PICK1 Polyclonal Antibody

Product Details

APZ-014-200UL

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

Immunohistochemistry (Frozen) (IHC (F))

1:400

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)RARFSQMRKDVLEK, corresponding to amino acid residues 305-318 of rat PICK1, Intracellular, alpha-helix domain

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKCalpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK1 with PKCalpha is highly dependent on the activation of the kinase. It appears that PICK1 directs the activated form of PKCalpha to membrane anchored GluR2. Once phosphorylated by PKCalpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK1-dependent manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC15204; OTTHUMP00000028509; PRKCA-binding protein; Protein interacting with C kinase 1; protein kinase C, alpha binding protein; Protein kinase C-alpha-binding protein; protein that interacts with C kinase 1

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Gene Aliases: PICK; PICK1; PRKCABP

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UniProt ID: (Human) Q9NRD5, (Rat) Q9EP80

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Entrez Gene ID: (Human) 9463, (Mouse) 18693, (Rat) 84591

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Function(s)

G-protein coupled receptor binding protein kinase C binding receptor binding protein binding protein C-terminus binding ATPase activity enzyme binding protein domain specific binding identical protein binding metal ion binding actin filament binding Arp2/3 complex binding actin binding kinase activity receptor tyrosine kinase binding G-protein coupled glutamate receptor binding ephrin receptor binding GTPase binding

Process(es)

positive regulation of receptor internalization protein phosphorylation protein targeting protein kinase C-activating G-protein coupled receptor signaling pathway multicellular organism development negative regulation of gene expression monoamine transport glial cell development regulation of Arp2/3 complex-mediated actin nucleation negative regulation of Arp2/3 complex-mediated actin nucleation intracellular signal transduction cellular response to decreased oxygen levels cellular response to glucose starvation receptor clustering regulation of synaptic plasticity regulation of synapse structure or activity protein homooligomerization long term synaptic depression negative regulation of cell death dendritic spine organization dendritic spine maintenance dopamine transport phosphorylation retrograde vesicle-mediated transport, Golgi to ER DNA methylation involved in embryo development DNA methylation involved in gamete generation neuronal ion channel clustering

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PICK1 Polyclonal Antibody

PICK1 Antibody (APZ-014-200UL) in IHC (F)

Product Details

APZ-014-200UL

Western Blot (WB)

Immunohistochemistry (Frozen) (IHC (F))

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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