Invitrogen

PHGDH Monoclonal Antibody (OTI5C4)

Name: PHGDH Monoclonal Antibody (OTI5C4)
Brand: Invitrogen
SKU: MA5-26812

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View all (33) PHGDH antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Advanced Verification (1)

PHGDH Antibody in Immunocytochemistry (ICC/IF)

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PHGDH Antibody (MA5-26812) in ICC/IF

Immunofluorescence analysis of PHGDH was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PHGDH Monoclonal Antibody (OTI5C4) (Product # MA5-26812) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More

PHGDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PHGDH Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of D-3-phosphoglycerate dehydrogenase was observed across the panel tested as compared to A549, MDA-MB-231, and SK-O-V3 which showed low to negative expression as reported in the literature using Anti-PHGDH Monoclonal Antibody (OTI5C4) (Product # MA5-26812) in Western Blot. {RE}

Immunogen image

Immunogen Image PHGDH Monoclonal Antibody (OTI5C4)

Product Details

MA5-26812

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI5C4

Immunogen

Full length human recombinant protein of PHGDH produced in HEK293T cell

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2725014

Target Information

PHGDH gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2-oxoglutarate reductase; 3-PGDH; 3-phosphoglycerate dehydrogenase; 3-phosphoglycerate dehyrogenase; A10; D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; Malate dehydrogenase

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Gene Aliases: 3-PGDH; 3PGDH; 4930479N23; A10; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PGDH3; PHGDH; PHGDHD; SERA

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UniProt ID: (Human) O43175, (Mouse) Q61753, (Rat) O08651

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Entrez Gene ID: (Human) 26227, (Mouse) 236539, (Rat) 58835

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Function(s)

phosphoglycerate dehydrogenase activity electron carrier activity NAD binding oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Process(es)

glutamine metabolic process glycine metabolic process L-serine biosynthetic process threonine metabolic process brain development serine family amino acid biosynthetic process gamma-aminobutyric acid metabolic process regulation of gene expression taurine metabolic process spinal cord development glial cell development neural tube development neuron projection development oxidation-reduction process G1 to G0 transition L-serine metabolic process metabolic process cellular amino acid biosynthetic process neurogenesis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PHGDH Monoclonal Antibody (OTI5C4)

PHGDH Antibody (MA5-26812) in ICC/IF

Product Details

MA5-26812

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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