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  • PHGDH Antibodies

Invitrogen

PHGDH Polyclonal Antibody

View all (33) PHGDH antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite PHGDH Polyclonal Antibody

  • Antibody Testing Data (2)
PHGDH Antibody in Western Blot (WB)
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PHGDH Antibody in Western Blot (WB)
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PHGDH Antibody (PA5-80896) in WB

Western Blot using PHGDH Polyclonal Antibody (Product # PA5-80896) at 1:500 dilution. Lane A: Hela Whole Cell Lysate, Lane B: A431 Whole Cell Lysate, Lane C: Raji Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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PHGDH Antibody in Western Blot (WB)
PHGDH Antibody in Immunoprecipitation (IP)
PHGDH Polyclonal Antibody

Product Details

PA5-80896

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

ELISA (ELISA)

1:5,000-1:10,000
-

Immunoprecipitation (IP)

4-8 µL/mg of lysate
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human PGDH/PHGDH Protein (Met1-Phe533)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2788160

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Human PGDH/PHGDH.

Target Information

PHGDH gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2-oxoglutarate reductase; 3-PGDH; 3-phosphoglycerate dehydrogenase; D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; Malate dehydrogenase

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Gene Aliases: 3-PGDH; 3PGDH; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PGDH3; PHGDH; PHGDHD; SERA

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UniProt ID: (Human) O43175

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Entrez Gene ID: (Human) 26227

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Function(s)
phosphoglycerate dehydrogenase activity electron carrier activity NAD binding
Process(es)
glutamine metabolic process glycine metabolic process L-serine biosynthetic process threonine metabolic process brain development serine family amino acid biosynthetic process gamma-aminobutyric acid metabolic process regulation of gene expression taurine metabolic process spinal cord development glial cell development neural tube development neuron projection development oxidation-reduction process G1 to G0 transition
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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