Invitrogen

PHGDH Polyclonal Antibody

Name: PHGDH Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-27579

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Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (6)
Advanced Verification (1)

PHGDH Antibody in Immunocytochemistry (ICC/IF)

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PHGDH Antibody (PA5-27579) in ICC/IF

Immunofluorescence analysis of PHGDH was performed using 70% confluent log phase NTERA-2 cl.D1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD2AP Polyclonal Antibody (Product # PA5-27579) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731) a... View More

PHGDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PHGDH Antibody in Immunohistochemistry (Frozen) (IHC (F))

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PHGDH Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of D-3-phosphoglycerate dehydrogenase was observed in all the models except in A549, MDA-MB-231 and SKOV3 which are reported to be negative using Anti-PHGDH Polyclonal Antibody (Product # PA5-27579) in Western Blot. {RE}

Product Details

PA5-27579

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHGDH. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545055

Product Specific Information

Recommended positive controls: 293T, A431, Jurkat, Raji, HeLa.

Predicted reactivity: Mouse (96%), Rat (97%), Pig (90%), Chimpanzee (100%), Bovine (83%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PHGDH gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 2-oxoglutarate reductase; 3-PGDH; 3-phosphoglycerate dehydrogenase; 3-phosphoglycerate dehyrogenase; A10; D-3-phosphoglycerate dehydrogenase; epididymis secretory protein Li 113; Malate dehydrogenase

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Gene Aliases: 3-PGDH; 3PGDH; 4930479N23; A10; HEL-S-113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PGDH3; PHGDH; PHGDHD; SERA

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UniProt ID: (Human) O43175, (Mouse) Q61753, (Rat) O08651

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Entrez Gene ID: (Human) 26227, (Mouse) 236539, (Rat) 58835

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Function(s)

phosphoglycerate dehydrogenase activity electron carrier activity NAD binding oxidoreductase activity oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Process(es)

glutamine metabolic process glycine metabolic process L-serine biosynthetic process threonine metabolic process brain development serine family amino acid biosynthetic process gamma-aminobutyric acid metabolic process regulation of gene expression taurine metabolic process spinal cord development glial cell development neural tube development neuron projection development oxidation-reduction process G1 to G0 transition L-serine metabolic process metabolic process cellular amino acid biosynthetic process neurogenesis

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PHGDH Polyclonal Antibody

PHGDH Antibody (PA5-27579) in ICC/IF

Product Details

PA5-27579

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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