Invitrogen

PHF14 Polyclonal Antibody

Name: PHF14 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-72775

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1 Reference

View all (4) PHF14 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (2)
Advanced Verification (1)

PHF14 Antibody in Immunocytochemistry (ICC/IF)

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PHF14 Antibody (PA5-72775) in ICC/IF

Immunofluorescence of PHF14 in 293 cells with PHF14 Polyclonal Antibody (Product # PA5-72775) at 5 µg/mL.

Product Image Gallery

PHF14 Antibody — PHF14 silencing in glioma cell lines decelerates proliferation and clonogenic growth. a, b The knockdown efficacy of PHF14 examined in U251, U87MG and A172 cells by qRT-PCR (a) and Western blot (b). c, d Cellular DNA replication is mitigated after PHF14 knocked down. Ten thousand U87MG, U251 and A172 cells per well were seeded in 96-well plates overnight. EdU and Hoechest co-staining was performed on the second day to assess cellular DNA replication activities (c) (Scale bar=100 µm). Values are counted as EdU+ cells/total cells (d). e-g PHF14 silenced cells showed weaker proliferation and colony formation potential. 500 U251 and A172 cells per well were cultured in 6-well plates in Complete growth medium for 10 days and then stained by 1% crystal violet for colony formation assay (e) and single colony morphology (f) (Scale bar=200 µm). The number of colonies containing over 50 cells were counted up for analysis (g). h Cell colonies were stained by Phalloidin-cy3 after 10 days of culture for assessment of actin filaments containment and cellular protrusions (Scale bar=50 µm) (*P<0.05; **P=0.01; ***P<0.001; ****P<0.0001) {KD}

Immunogen image

Immunogen Image PHF14 Polyclonal Antibody

Product Details

PA5-72775

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PHF14 antibody for a 19 amino acid peptide near the amino terminus of human PHF14. The immunogen is within amino acids 210 - 260 of PHF14.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2718629

Target Information

PHF14 gene ontology annotations related to this gene include metal ion binding.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: PHD finger protein 14

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Gene Aliases: 1110001C23Rik; 4932409F11Rik; 5730446A07Rik; AA623952; AV297001; KIAA0783; mKIAA0783; PHF14; RGD1563764

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UniProt ID: (Human) O94880, (Mouse) Q9D4H9

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Entrez Gene ID: (Human) 9678, (Mouse) 75725, (Rat) 500030

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Function(s)

zinc ion binding molecular_function metal ion binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter negative regulation of cell proliferation lung alveolus development negative regulation of mesenchymal cell proliferation negative regulation of platelet-derived growth factor receptor-alpha signaling pathway negative regulation of mesenchymal cell proliferation involved in lung development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PHF14 Polyclonal Antibody

PHF14 Antibody (PA5-72775) in ICC/IF

Product Details

PA5-72775

Western Blot (WB)

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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