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  • Primary Antibodies ›
  • PHD2 Antibodies

Invitrogen

PHD2 Polyclonal Antibody

View all (18) PHD2 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite PHD2 Polyclonal Antibody

  • Antibody Testing Data (2)
PHD2 Antibody in Western Blot (WB)
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PHD2 Antibody in Western Blot (WB)
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PHD2 Antibody (PA5-119588) in WB

Western blot analysis of PHD2 in wild-type (WT) and PHD2 knockout (KO) 293T cell extracts (30 µg). Samples were incubated with PHD2 polyclonal antibody (Product # PA5-119588) using a dilution of 1:500 followed by HRP-conjugated anti-rabbit IgG. Separated by 10% SDS-PAGE. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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PHD2 Antibody in Western Blot (WB)
PHD2 Antibody in Western Blot (WB)

Product Details

PA5-119588

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHD2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.89 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2913161

Product Specific Information

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Information

SYMPK is a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C1ORF12 antibody; Egl nine homolog 1; egl nine-like protein 1; egl-9 family hypoxia-inducible factor 1; EGLN 1 EGLN1; HIF PH2; HIF prolyl hydroxylase 2; HIF-P4H 2; HIF-PH2; HIF-prolyl hydroxylase 2; Hypoxia-inducible factor prolyl hydroxylase 2; P4H2; PHD2; Prolyl Hy; Prolyl hydroxylase domain-containing protein 2; SM-20; zinc finger MYND domain-containing protein 6

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Gene Aliases: C1orf12; ECYT3; EGLN1; HALAH; HIF-PH2; HIFPH2; HPH-2; HPH2; PHD2; PNAS-118; PNAS-137; SM20; ZMYND6

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UniProt ID: (Human) Q9GZT9

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Entrez Gene ID: (Human) 54583

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Function(s)
iron ion binding protein binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors enzyme binding L-ascorbic acid binding peptidyl-proline dioxygenase activity peptidyl-proline 4-dioxygenase activity
Process(es)
response to hypoxia peptidyl-proline hydroxylation to 4-hydroxy-L-proline negative regulation of cAMP catabolic process oxygen homeostasis negative regulation of sequence-specific DNA binding transcription factor activity regulation of angiogenesis negative regulation of cyclic-nucleotide phosphodiesterase activity cardiac muscle tissue morphogenesis oxidation-reduction process heart trabecula formation ventricular septum morphogenesis labyrinthine layer development regulation of transcription from RNA polymerase II promoter in response to hypoxia response to nitric oxide
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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