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  • Primary Antibodies ›
  • PHD2 Antibodies

Invitrogen

PHD2 Polyclonal Antibody

View all (18) PHD2 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite PHD2 Polyclonal Antibody

  • Antibody Testing Data (4)
PHD2 Antibody in Immunocytochemistry (ICC/IF)
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PHD2 Antibody in Immunocytochemistry (ICC/IF)
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PHD2 Antibody (PA1-16946) in ICC/IF

Immunocytochemistry analysis of PHD2 in HeLa cells. Samples were incubated with PHD2 polyclonal antibody (Product # PA1-16946) using a dilution of 1:500 followed by Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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PHD2 Antibody in Immunocytochemistry (ICC/IF)
PHD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
PHD2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
PHD2 Antibody in Western Blot (WB)
PHD2 Polyclonal Antibody

Product Details

PA1-16946

Applications
Tested Dilution
Publications

Western Blot (WB)

2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2.5-5.0 µg/mL
-

Immunocytochemistry (ICC/IF)

1:50-1:500
-

Immunoprecipitation (IP)

1:10-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide made to an internal portion of mouse PHD2/HIF Prolyl Hydroxylase 2 (between residues 300-400).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2277703

Product Specific Information

This antibody is expected to react with rat based on sequence homology.

Target Information

SYMPK is a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.This gene encodes a nuclear protein that functions in the regulation of polyadenylation and promotes gene expression. The protein forms a high-molecular weight complex with components of the polyadenylation machinery. It is thought to serve as a scaffold for recruiting regulatory factors to the polyadenylation complex. It also participates in 3'-end maturation of histone mRNAs, which do not undergo polyadenylation. The protein also localizes to the cytoplasmic plaques of tight junctions in some cell types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C1ORF12 antibody; Egl nine homolog 1; egl nine-like protein 1; egl-9 family hypoxia-inducible factor 1; EGLN 1 EGLN1; HIF PH2; HIF prolyl hydroxylase 2; HIF-P4H 2; HIF-PH2; HIF-prolyl hydroxylase 2; Hypoxia-inducible factor prolyl hydroxylase 2; P4H2; PHD2; Prolyl Hy; Prolyl hydroxylase domain-containing protein 2; SM-20; zinc finger MYND domain-containing protein 6

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Gene Aliases: AI503754; C1orf12; ECYT3; EGLN1; HALAH; Hif-p4h-2; HIF-PH2; HIFPH2; HPH-2; HPH2; ORF13; PHD-2; PHD2; PNAS-118; PNAS-137; SM-20; SM20; ZMYND6

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UniProt ID: (Human) Q9GZT9, (Mouse) Q91YE3

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Entrez Gene ID: (Human) 54583, (Mouse) 112405, (Rat) 308913

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Function(s)
iron ion binding protein binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors enzyme binding L-ascorbic acid binding peptidyl-proline dioxygenase activity peptidyl-proline 4-dioxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding dioxygenase activity
Process(es)
response to hypoxia peptidyl-proline hydroxylation to 4-hydroxy-L-proline negative regulation of cAMP catabolic process oxygen homeostasis negative regulation of sequence-specific DNA binding transcription factor activity regulation of angiogenesis negative regulation of cyclic-nucleotide phosphodiesterase activity cardiac muscle tissue morphogenesis oxidation-reduction process heart trabecula formation ventricular septum morphogenesis labyrinthine layer development response to nitric oxide regulation of transcription from RNA polymerase II promoter in response to hypoxia
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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