Invitrogen

PDHA1 Monoclonal Antibody (8D10E6)

Name: PDHA1 Monoclonal Antibody (8D10E6)
Brand: Invitrogen
SKU: 45-6600

Advanced Verification

4 Published Figures

6 References

View all (32) PDHA1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

PDHA1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 9

PDHA1 Antibody (45-6600) in ICC/IF

Immunofluorescent analysis of PDH in HeLa cells using a PDH Monoclonal antibody (Product # 45-6600) at 1 µg/mL. Samples were fixed with 4% paraformaldehyde and permeabilized with Triton X-100. The secondary antibody was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution, seen in green. DAPI was used to stain the cell nuclei as seen in blue.

Product Image Gallery

PDHA1 Antibody — Figure 2 DCA recovers mitochondrial respiration rate and controls proliferation in SOD1 G93A astrocytes. A) Representative immunoblot for PDH-E1alpha(pSer 293 ), total PDH-E1alpha, and beta-actin of lysates from non Tg and SOD1 G93A astrocytes after 24 h treatment with DCA or vehicle as described in Methods . B) Quantification of the PDH-E1alpha(pSer 293 ) to total PDH-E1alpha ratio between relative densitometric levels normalized against vehicle-treated non Tg astrocytes. C) Calculated respiratory control ratio (RCR) for mitochondria from non Tg or SOD1 G93A -bearing astrocytes treated with DCA or vehicle as indicated. D) Percentage of BrdU immunoreactive nuclei of non Tg and SOD1 G93A astrocytes after 24 h treatment with DCA. Data for panels B, C, and D are expressed as mean +- SEM from three independent experiments performed in duplicate. *p<0.05, significantly different from non Tg control. **p<0.05, significantly different from SOD1 G93A control. {TM}

Product Details

45-6600

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1.0 µg/mL

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1 µg/mL

Flow Cytometry (Flow)

1 µg/mL

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Fruit fly, Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

8D10E6

Immunogen

Human recombinant PDH-E1-alpha

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

IgG fraction

Storage buffer

HEPES buffered saline

Contains

0.02% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533825

Product Specific Information

When performing ICC and IF heat-induced antigen-retrieval improves signal.

When performing Flow Cytometry Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Positive Control: WB: Isolated mitochondria from human, bovine, rat and mouse heart. HepG2 (human liver hepatocellular carcinoma cell line) cell lysate. FACS: HeLa (human epithelial cell line from cervix adenocarcinoma) and HL-60 (human promyelocytic leukemia cell line) cells.

Target Information

The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: PDHE1-A type I; pyruvate dehydrogenase; pyruvate dehydrogenase complex, E1-alpha polypeptide 1; pyruvate dehydrogenase E1 alpha 1; pyruvate dehydrogenase E1 component alpha subunit; Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; pyruvate dehydrogenase E1 subunit; pyruvate dehydrogenase E1alpha subunit

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Gene Aliases: BOS_26312; PDHA; Pdha-1; PDHA1; PDHAD; PDHCE1A; PHE1A

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UniProt ID: (Human) P08559, (Bovine) A7MB35, (Mouse) P35486

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Entrez Gene ID: (Human) 5160, (Bovine) 407109, (Rat) 29554, (Mouse) 18597

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Function(s)

pyruvate dehydrogenase activity pyruvate dehydrogenase (acetyl-transferring) activity pyruvate dehydrogenase (NAD+) activity oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor

Process(es)

glucose metabolic process acetyl-CoA biosynthetic process from pyruvate glycolytic process tricarboxylic acid cycle oxidation-reduction process carbohydrate metabolic process metabolic process pyruvate metabolic process regulation of acetyl-CoA biosynthetic process from pyruvate glyoxylate metabolic process mitochondrial acetyl-CoA biosynthetic process from pyruvate

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PDHA1 Monoclonal Antibody (8D10E6)

PDHA1 Antibody (45-6600) in ICC/IF

Product Details

45-6600

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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