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  • Protease-Activated Receptor-4 Antibodies

Alomone Labs, LTD

PAR4 (F2RL3) (extracellular) Polyclonal Antibody

View all (9) Protease-Activated Receptor-4 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite PAR4 (F2RL3) (extracellular) Polyclonal Antibody

  • Antibody Testing Data (6)
PAR4 (F2RL3) (extracellular) Antibody in Immunocytochemistry (ICC/IF)
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PAR4 (F2RL3) (extracellular) Antibody in Immunocytochemistry (ICC/IF)
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PAR4 (F2RL3) (extracellular) Antibody (APR-034-200UL) in ICC/IF

Expression of PAR-4 in a human breast adenocarcinoma cell line - Cell surface detection of PAR-4 in a human breast adenocarcinoma cell line. A. Live intact human MCF-7 cells were stained with Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:50), followed by goat Anti-rabbit-AlexaFluor-488 secondary Antibody (green). B. Live view of the cells. C. Nuclei were visualized with the cell-permeable dye Ho... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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PAR4 (F2RL3) (extracellular) Antibody in Immunocytochemistry (ICC/IF)
PAR4 (F2RL3) (extracellular) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
PAR4 (F2RL3) (extracellular) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
PAR4 (F2RL3) (extracellular) Antibody in Western Blot (WB)
PAR4 (F2RL3) (extracellular) Antibody in Western Blot (WB)
PAR4 (F2RL3) (extracellular) Antibody in Flow Cytometry (Flow)
PAR4 (F2RL3) (extracellular) Polyclonal Antibody

Product Details

APR-034-200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100
-

Immunocytochemistry (ICC/IF)

1:50
-

Flow Cytometry (Flow)

2.5 µg
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)HLRGQRWPFGEAA(S)R, corresponding to amino acid residues 136-150 of human PAR-4, 1stextracellular loop
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Coagulation factor II (thrombin) receptor-like 3 (F2RL3) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL3 is also a member of the protease-activated receptor family. F2RL3 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL3 is activated by thrombin and trypsin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: coagulation factor II (thrombin) receptor-like 3; Coagulation factor II receptor-like 3; PAR-4; protease-activated receptor 4; protease-activated receptor-4; Proteinase-activated receptor 4; Thrombin receptor-like 3

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Gene Aliases: F2RL3; PAR4

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UniProt ID: (Human) Q96RI0, (Rat) Q920E0, (Mouse) O88634

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Entrez Gene ID: (Human) 9002, (Rat) 116498, (Mouse) 14065

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Function(s)
G-protein coupled receptor activity thrombin receptor activity signal transducer activity
Process(es)
blood coagulation positive regulation of release of sequestered calcium ion into cytosol thrombin receptor signaling pathway signal transduction G-protein coupled receptor signaling pathway hemostasis phospholipase C-activating G-protein coupled receptor signaling pathway response to wounding platelet activation platelet dense granule organization
It has to be done as per old AB suggested Products section.
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