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  • P4HA1 Antibodies

Invitrogen

P4HA1 Polyclonal Antibody

Advanced Verification
View all (9) P4HA1 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite P4HA1 Polyclonal Antibody

  • Antibody Testing Data (6)
  • Advanced Verification (1)
P4HA1 Antibody in Immunocytochemistry (ICC/IF)
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P4HA1 Antibody in Immunocytochemistry (ICC/IF)
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P4HA1 Antibody (PA5-52454) in ICC/IF

Immunofluorecent analysis of P4HA1 in human cell line U-251 MG using P4HA1 Polyclonal Antibody (Product # PA5-52454). Staining shows localization to endoplasmic reticulum. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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P4HA1 Antibody in Immunocytochemistry (ICC/IF)
P4HA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
P4HA1 Antibody in Immunohistochemistry (IHC)
P4HA1 Antibody in Immunohistochemistry (IHC)
P4HA1 Antibody in Immunohistochemistry (IHC)
P4HA1 Antibody in Western Blot (WB)
P4HA1 Antibody
P4HA1 Polyclonal Antibody

Product Details

PA5-52454

Applications
Tested Dilution
Publications

Western Blot (WB)

0.04-0.4 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500
-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human P4HA1. Recombinant protein control fragment (Product #RP-89690).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2645146

Product Specific Information

Immunogen sequence: FTSIGQMTDL IHTEKDLVTS LKDYIKAEED KLEQIKKWAE KLDRLTSTAT KDPEGFVGHP VNAFKLMKRL NTEWSELENL VLKDMSDGFI SNLTIQRQYF PNDEDQVGAA KALLRLQDTY NLDTDTISKG NLPGVKHKSF LTAEDC

Highest antigen sequence identity to the following orthologs: Mouse - 97%, Rat - 97%.

Target Information

This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 4-PH alpha-1; C-P4Halpha(I); collagen prolyl 4-hydroxylase alpha(I); procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; Procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1; Prolyl 4-hydroxylase subunit alpha-1; prolyl 4-hydroxylase, alpha polypeptide I

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Gene Aliases: P4HA; P4HA1

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UniProt ID: (Human) P13674

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Entrez Gene ID: (Human) 5033

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Function(s)
procollagen-proline 4-dioxygenase activity iron ion binding protein binding oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen L-ascorbic acid binding
Process(es)
peptidyl-proline hydroxylation to 4-hydroxy-L-proline collagen fibril organization oxidation-reduction process
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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