Invitrogen

P-Selectin Polyclonal Antibody

Name: P-Selectin Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-81578

View all (58) P-Selectin (CD62P) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

P-Selectin Antibody in Western Blot (WB)

FIGURE: 1 / 1

P-Selectin Antibody (PA5-81578) in WB

Western Blot using P-Selectin Polyclonal Antibody (Product # PA5-81578) at 1:500 dilution. Lane A: K562 Whole Cell Lysate, Lane B: 293T Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 91 kDa. Observed band size: 91 kDa. (We are unsure of the identity of these extra bands).

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Product Details

PA5-81578

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

ELISA (ELISA)

1:25,000-1:50,000

Product Specifications

Species Reactivity

Rhesus monkey

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Rhesus SELP/P Selectin protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2788776

Product Specific Information

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).

This antibody has specificity for Rhesus SELP/P Selectin.

Target Information

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ45155; RP1-86F14.2; selectin P (granule membrane protein 140kDa, antigen CD62)

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Entrez Gene ID: (Rhesus monkey) 701277