Invitrogen

Oct-2 Monoclonal Antibody (ZO001)

Name: Oct-2 Monoclonal Antibody (ZO001)
Brand: Invitrogen
SKU: 39-5400

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3 Published Figures

5 References

View all (26) Oct-2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

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FIGURE: 1 / 8

Oct-2 Antibody (39-5400) in WB

Western blot analysis was performed on nuclear enriched extracts (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2), NTERA-2 (Lane 3), MOLT4 (Lane 4), NCCIT (Lane 5), A549 (Lane 6), HeLa (Lane 7), HEK-293 (Lane 8) and Jurkat (Lane 9). The blot was probed with Anti- Oct-2 Mouse Monoclonal Antibody (Product # 39-5400, 1 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 62 kDa band corresponding to Oct-2 was observed in Raji, ... View More

Oct-2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

Oct-2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of Oct-2 was observed in Raji, Ramos, NTERA-2, MOLT4, NCCIT , A549, HeLa, HEK-293 and Jurkat in Western Blot using Oct-2 Monoclonal Antibody (Product # 39-5400). Oct-2 is reported to be expressed in B lymphocytes (Raji, Ramos) and not in other cell lines like NTERA-2, MOLT4, NCCIT , A549, HeLa, HEK-293 and Jurkat. {RE}

Immunogen image

Immunogen Image Oct-2 Monoclonal Antibody (ZO001)

Product Details

39-5400

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ZO001

Immunogen

Synthetic peptide derived from the C-terminus of human Oct2.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533419

Target Information

OCT-2 is a transcription factor belonging to the POU homeo-domain family that binds to the Ig gene octamer sites regulating B cell specific genes. OCT-2 protein expression is not restricted to B cells, although expression levels are much higher in these cells. OCT-2 expression is reported to be significantly greater in germinal center derived lymphomas, although other B cell lymphomas also display high levels of expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: homeobox protein; Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; Oct-2; Octamer-binding protein 2; Octamer-binding transcription factor 2; OTF-2; POU domain, class 2, transcription factor 2

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Gene Aliases: Oct-2; OCT2; OTF2; POU2F2

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UniProt ID: (Human) P09086

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Entrez Gene ID: (Human) 5452

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein domain specific binding sequence-specific DNA binding

Process(es)

mature B cell differentiation immunoglobulin secretion involved in immune response transcription from RNA polymerase II promoter humoral immune response snRNA transcription from RNA polymerase II promoter positive regulation of transcription from RNA polymerase II promoter cell maturation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Oct-2 Monoclonal Antibody (ZO001)

Oct-2 Antibody (39-5400) in WB

Product Details

39-5400

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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