Invitrogen

OGG1 Polyclonal Antibody

Name: OGG1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-16505

1 Reference

View all (16) OGG1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)

OGG1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

OGG1 Antibody (PA1-16505) in ICC/IF

Immunocytochemistry analysis of OGG1 in A431 cells. Samples were incubated with OGG1 polyclonal antibody (Product # PA1-16505) using a dilution of 1:200 dilution overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. Alpha Tubulin (DM1A) was used as a co-stain at a 1:1,000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Bl... View More

OGG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image OGG1 Polyclonal Antibody

Product Details

PA1-16505

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:100

Immunocytochemistry (ICC/IF)

1:100-1:200

Flow Cytometry (Flow)

Assay-Dependent

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide derived from human Ogg1 (within amino acids 1-100).

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2156791

Product Specific Information

Suggested positive control: Jurkat whole cell extracts.

Target Information

8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. In E. Coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue. hOgg1 proteins efficiently released the 8-hydroxyguanine opposite the pyrimidine from DNA and cleaved the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development. The hOgg1 gene has been mapped to region 3p26. 2, a region showing loss of heterozygosity (LOH) in a variety of cancers. In particular, 3p25-p26 is a common LOH region in lung cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 8-hydroxyguanine DNA glycosylase; AP lyase; DNA-apurinic or apyrimidinic site lyase; N-glycosylase/DNA lyase; OGG1 type 1f

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Gene Aliases: HMMH; HOGG1; MMH; MUTM; OGG1; OGH1

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UniProt ID: (Human) O15527, (Mouse) O08760

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Entrez Gene ID: (Human) 4968, (Mouse) 18294

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Function(s)

damaged DNA binding endonuclease activity protein binding microtubule binding oxidized purine nucleobase lesion DNA N-glycosylase activity oxidized purine DNA binding 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity purine-specific mismatch base pair DNA N-glycosylase activity oxidized base lesion DNA N-glycosylase activity DNA binding catalytic activity DNA-(apurinic or apyrimidinic site) lyase activity uracil DNA N-glycosylase activity hydrolase activity hydrolase activity, acting on glycosyl bonds lyase activity single-strand selective uracil DNA N-glycosylase activity DNA N-glycosylase activity DNA-3-methylbase glycosylase activity nicotinamide riboside hydrolase activity nicotinic acid riboside hydrolase activity deoxyribonucleoside 5'-monophosphate N-glycosidase activity

Process(es)

acute inflammatory response base-excision repair regulation of transcription, DNA-templated cellular response to DNA damage stimulus response to oxidative stress aging response to radiation response to light stimulus response to estradiol regulation of protein import into nucleus, translocation nucleotide-excision repair, DNA incision response to drug negative regulation of apoptotic process depurination depyrimidination response to ethanol response to folic acid cellular response to cadmium ion negative regulation of double-strand break repair via single-strand annealing DNA repair nucleotide-excision repair metabolic process nucleic acid phosphodiester bond hydrolysis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OGG1 Polyclonal Antibody

OGG1 Antibody (PA1-16505) in ICC/IF

Product Details

PA1-16505

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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