Invitrogen

OGDH Polyclonal Antibody

Name: OGDH Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-28195

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2 Published Figures

3 References

View all (11) OGDH antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

OGDH Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

OGDH Antibody (PA5-28195) in ICC/IF

Immunofluorescence analysis of OGDH was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with OGDH Polyclonal Antibody (Product # PA5-28195) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2703... View More

OGDH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

OGDH Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep G2 cells were transfected with OGDH siRNA and reduction of signal was observed in Western Blot using OGDH Polyclonal Antibody (Product # PA5-28195). {KD}

Immunogen image

Immunogen Image OGDH Polyclonal Antibody

Product Details

PA5-28195

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:10,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human OGDH. The exact sequence is proprietary.

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2545671

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, Huh-7, Mouse brain.

Predicted reactivity: Mouse (93%), Rat (93%), Xenopus laevis (84%), Chicken (87%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitocondrial matrix and uses thiamine pyrophosphate as a cofactor. A congential deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 2-oxoglutarate dehydrogenase complex component E1; 2-oxoglutarate dehydrogenase, mitochondrial; Alpha-ketoglutarate dehydrogenase; Alpha-KGDH-E1; E1o; OGDC-E1; oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide); oxoglutarate decarboxylase; oxoglutarate dehydrogenase (lipoamide); oxoglutarate dehydrogenase (succinyl-transferring); testicular tissue protein Li 131; Thiamine diphosphate (ThDP)-dependent 2-oxoglutarate dehydrogenase

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Gene Aliases: 2210403E04Rik; 2210412K19Rik; AA409584; AKGDH; d1401; E1k; Kiaa4192; mKIAA4192; OGDC; OGDH

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UniProt ID: (Human) Q02218, (Mouse) Q60597

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Entrez Gene ID: (Human) 4967, (Mouse) 18293

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Function(s)

oxoglutarate dehydrogenase (succinyl-transferring) activity thiamine pyrophosphate binding heat shock protein binding oxoglutarate dehydrogenase (NAD+) activity metal ion binding chaperone binding oxidoreductase activity oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor

Process(es)

generation of precursor metabolites and energy glycolytic process tricarboxylic acid cycle 2-oxoglutarate metabolic process succinyl-CoA metabolic process lysine catabolic process NADH metabolic process cerebellar cortex development striatum development hippocampus development thalamus development pyramidal neuron development tangential migration from the subventricular zone to the olfactory bulb glyoxylate metabolic process olfactory bulb mitral cell layer development metabolic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OGDH Polyclonal Antibody

OGDH Antibody (PA5-28195) in ICC/IF

Product Details

PA5-28195

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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