Invitrogen

OAS2 Monoclonal Antibody (OTI5E1)

Name: OAS2 Monoclonal Antibody (OTI5E1)
Brand: Invitrogen
SKU: MA5-26557

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View all (32) OAS2 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (5)
Advanced Verification (2)

OAS2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 7

OAS2 Antibody (MA5-26557) in ICC/IF

Immunofluorescence analysis of 2-5-oligoadenylate synthase 2 was performed using 70% confluent log phase A549 cells treated with IFN beta 1A (1000 U/mL, O/N). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with OAS2 Monoclonal Antibody (OTI5E1) (Product # MA5-26557) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsor... View More

OAS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

OAS2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using OAS2 Monoclonal Antibody (OTI5E1) (Product # MA5-26557), shows upregulation in OAS2 expression upon IFN beta 1A treatment in A549, and IFN alpha A treatment in HT-1080 and HeLa (https://doi.org/10.1073/pnas.1519657113). {TM}

Immunogen image

Immunogen Image OAS2 Monoclonal Antibody (OTI5E1)

Product Details

MA5-26557

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Immunocytochemistry (ICC/IF)

1:200

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI5E1

Immunogen

Human recombinant protein fragment corresponding to amino acids 60-335 of OAS2 produced in E.coli

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2724897

Target Information

This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates. These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Alternatively spliced transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: (2'-5')oligo(A) synthetase 2; (2-5')oligo(A) synthase 2; 2'-5'-oligoadenylate synthase 2; 2'-5'-oligoadenylate synthetase 2, 69/71kDa; 2-5A synthase 2; A synthetase 2; MGC78578; p69 OAS / p71 OAS; p69OAS / p71OAS

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Gene Aliases: OAS2

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UniProt ID: (Human) P29728

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Entrez Gene ID: (Human) 4939

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Function(s)

2'-5'-oligoadenylate synthetase activity double-stranded RNA binding protein binding ATP binding metal ion binding

Process(es)

nucleobase-containing compound metabolic process RNA catabolic process protein glycosylation response to virus protein myristoylation defense response to virus interferon-gamma-mediated signaling pathway type I interferon signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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OAS2 Monoclonal Antibody (OTI5E1)

OAS2 Antibody (MA5-26557) in ICC/IF

Product Details

MA5-26557

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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