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  • Nucleostemin Antibodies

Invitrogen

Nucleostemin Polyclonal Antibody

Advanced Verification
View all (36) Nucleostemin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Nucleostemin Polyclonal Antibody

  • Antibody Testing Data (8)
  • Advanced Verification (1)
Nucleostemin Antibody in Immunocytochemistry (ICC/IF)
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Nucleostemin Antibody in Immunocytochemistry (ICC/IF)
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Nucleostemin Antibody (PA5-83350) in ICC/IF

Immunofluorescence analysis of Guanine nucleotide-binding protein-like 3 was performed using 70% confluent log phase U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nucleostemin Polyclonal Antibody (Product # PA5-83350) at 1:100 dilution in... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Nucleostemin Antibody in Immunocytochemistry (ICC/IF)
Nucleostemin Antibody in Immunocytochemistry (ICC/IF)
Nucleostemin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nucleostemin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nucleostemin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nucleostemin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nucleostemin Antibody in Western Blot (WB)
Nucleostemin Antibody in Western Blot (WB)
Nucleostemin Antibody
Nucleostemin Polyclonal Antibody

Product Details

PA5-83350

Applications
Tested Dilution
Publications

Western Blot (WB)

0.04-0.4 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500
-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human GNL3. Recombinant protein control fragment (Product #RP-102742).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2790506

Product Specific Information

Immunogen sequence: SILFQSSGLT NGIIEEKDIH EELPKRKERK QEEREDDKDS DQETVDEEVD ENSSGMFAAE ETGEALSEET TAGEQSTRSF I

Target Information

Arylsulfatase F, also known as ARSF, is a 590 amino acid secretory protein that belongs to the sulfatase family of bone and cartilage matrix proteins. Arylsulfatase F uses calcium as a cofactor to catalyze reactions that are important in maintaining correct bone composition. The activity of Arylsulfatase F, unlike that of other family members, such as Arylsulfatase E, is not inhibited by warfarin. The gene encoding Arylsulfatase F maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner's syndrome, color blindness, hemophilia and Duchenne muscular dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E2-induced gene 3 protein; estradiol-induced nucleotide binding protein; guanine nucleotide binding protein-like 3 (nucleolar); Guanine nucleotide-binding protein-like 3; MGC800; NNP47; Novel nucleolar protein 47; Nucleolar GTP-binding protein 3; Nucleostemin

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Gene Aliases: C77032; E2IG3; GNL3; NNP47; NS

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UniProt ID: (Human) Q9BVP2

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Entrez Gene ID: (Human) 26354

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Function(s)
GTPase activity protein binding GTP binding poly(A) RNA binding
Process(es)
cell proliferation positive regulation of telomere maintenance positive regulation of protein sumoylation regulation of cell proliferation ribosome biogenesis positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to chromosome, telomeric region
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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