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  • Primary Antibodies ›
  • Nucleostemin Antibodies

Invitrogen

Nucleostemin Polyclonal Antibody

View all (36) Nucleostemin antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Nucleostemin Polyclonal Antibody

  • Antibody Testing Data (8)
Nucleostemin Antibody in Immunocytochemistry (ICC/IF)
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Nucleostemin Antibody in Immunocytochemistry (ICC/IF)
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Nucleostemin Antibody (PA5-143898) in ICC/IF

Immunocytochemistry analysis of Nucleostemin in HEP3B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in Nucleostemin Polyclonal antibody (Product # PA5-143898) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution a... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Nucleostemin Antibody in Immunocytochemistry (ICC/IF)
Nucleostemin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nucleostemin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nucleostemin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nucleostemin Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nucleostemin Antibody in Western Blot (WB)
Nucleostemin Antibody in Flow Cytometry (Flow)
Nucleostemin Antibody in Flow Cytometry (Flow)
Nucleostemin Polyclonal Antibody

Product Details

PA5-143898

Applications
Tested Dilution
Publications

Western Blot (WB)

0.25-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

2-5 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human Nucleostemin/GNL3 recombinant protein (Position: R45-V549).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3075112

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human K562 whole cell, human PC-3 whole cell, human Jurkat whole cell, rat C6 whole cell, mouse NIH/3T3 whole cell. IHC: human appendiceal adenocarcinoma tissue, human breast cancer tissue, human colonic adenocarcinoma tissue, human gall bladder adenosquamous carcinoma tissue. ICC/IF: HEP3B cell. Flow: Hela cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Arylsulfatase F, also known as ARSF, is a 590 amino acid secretory protein that belongs to the sulfatase family of bone and cartilage matrix proteins. Arylsulfatase F uses calcium as a cofactor to catalyze reactions that are important in maintaining correct bone composition. The activity of Arylsulfatase F, unlike that of other family members, such as Arylsulfatase E, is not inhibited by warfarin. The gene encoding Arylsulfatase F maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner's syndrome, color blindness, hemophilia and Duchenne muscular dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: E2-induced gene 3 protein; estradiol-induced nucleotide binding protein; guanine nucleotide binding protein-like 3 (nucleolar); Guanine nucleotide-binding protein-like 3; MGC800; NNP47; Novel nucleolar protein 47; Nucleolar GTP-binding protein 3; Nucleostemin

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Gene Aliases: C77032; E2IG3; GNL3; NNP47; NS

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UniProt ID: (Human) Q9BVP2, (Mouse) Q8CI11, (Rat) Q811S9

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Entrez Gene ID: (Human) 26354, (Mouse) 30877, (Rat) 290556

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Function(s)
GTPase activity protein binding GTP binding poly(A) RNA binding nucleotide binding
Process(es)
cell proliferation regulation of cell proliferation ribosome biogenesis positive regulation of pri-miRNA transcription from RNA polymerase II promoter metabolic process positive regulation of telomere maintenance positive regulation of protein sumoylation positive regulation of protein localization to chromosome, telomeric region
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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