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Nucleolin (Marker of Human Cells) Monoclonal Antibody (SPM614)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Nucleolin (Marker of Human Cells) Monoclonal Antibody (SPM614)

  • Antibody Testing Data (5)
Nucleolin (Marker of Human Cells) Antibody in Immunocytochemistry (ICC/IF)
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Nucleolin (Marker of Human Cells) Antibody in Immunocytochemistry (ICC/IF)
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Nucleolin (Marker of Human Cells) Antibody (4691-MSM1X-P0) in ICC/IF

Immunofluorescence analysis of PFA-fixed K562 cells stained with Nucleolin Mouse Monoclonal Antibody (SPM614) followed by goat anti-mouse IgG-CF488 (green). Membrane stained with phalloidin-CF640. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Nucleolin (Marker of Human Cells) Antibody in Immunocytochemistry (ICC/IF)
Nucleolin (Marker of Human Cells) Antibody in Immunocytochemistry (ICC/IF)
Nucleolin (Marker of Human Cells) Antibody in Immunocytochemistry (ICC/IF)
Nucleolin (Marker of Human Cells) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nucleolin (Marker of Human Cells) Antibody in Flow Cytometry (Flow)
Nucleolin (Marker of Human Cells) Monoclonal Antibody (SPM614)

Product Details

4691-MSM1X-P0

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL
-

Immunocytochemistry (ICC/IF)

1-4 µg/mL
-

Flow Cytometry (Flow)

0.5-1 µg/10^6 cells
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SPM614

Immunogen

Recombinant full-length human NCL protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Wet ice

Product Specific Information

Does not react with Mouse, Rat or Cow

Target Information

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: FLJ45706; FLJ59041; nucl antibody; Nucleolin; Protein C23

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Gene Aliases: C23; NCL

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UniProt ID: (Human) P19338

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Entrez Gene ID: (Human) 4691

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Function(s)
nucleotide binding single-stranded DNA binding RNA binding calcium ion binding protein binding protein C-terminus binding selenocysteine insertion sequence binding rRNA primary transcript binding telomeric DNA binding histone binding identical protein binding laminin binding poly(A) RNA binding ErbB-4 class receptor binding
Process(es)
angiogenesis endocytosis spermatogenesis positive regulation of tumor necrosis factor production negative regulation of apoptotic process positive regulation of transcription from RNA polymerase II promoter cellular response to lipopolysaccharide liver regeneration positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter regulation of rRNA processing positive regulation of interleukin-6 secretion
It has to be done as per old AB suggested Products section.
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