Invitrogen

NuMA Monoclonal Antibody (A3A2)

Name: NuMA Monoclonal Antibody (A3A2)
Brand: Invitrogen
SKU: MA5-36123

View all (31) NuMA antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (14)

NuMA Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

NuMA Antibody (MA5-36123) in ICC/IF

Immunocytochemical analysis of NuMA in MCF-7 cells using a NuMA monoclonal antibody (Product #MA5-36123). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Mouse IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI.

NuMA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image NuMA Monoclonal Antibody (A3A2)

Product Details

MA5-36123

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:50-1:100

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A3A2

Immunogen

Recombinant protein within Human NuMA aa 1-200

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2890338

Target Information

NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; Nuclear matrix protein-22; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen; structural nuclear protein

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Gene Aliases: NMP-22; NMP22; NUMA; NUMA1

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UniProt ID: (Human) Q14980

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Entrez Gene ID: (Human) 4926

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Function(s)

structural molecule activity protein binding microtubule binding

Process(es)

establishment of mitotic spindle orientation nucleus organization mitotic nuclear division cell division meiotic cell cycle lung epithelial cell differentiation

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NuMA Monoclonal Antibody (A3A2)

NuMA Antibody (MA5-36123) in ICC/IF

Product Details

MA5-36123

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

References (0)

Bioinformatics

Performance Guarantee

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