Invitrogen

Nkx2.5 Polyclonal Antibody

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Datasheet
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Datasheet
Protocols
Questions & Answers
Nkx2.5 Polyclonal Antibody

Product Details

PA5-95225

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Nkx2.5 (103-132aa AKDPRAEKKELCALQKAVELEKTEADNAER).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2807029

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: Mouse Spleen Tissue, Mouse Cardiac Muscle Tissue, HELA whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cardiac-specific homeobox; cardiac-specific homeobox 1; Drosophila NK2 transcription factor related, locus 5; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein NKX 2-5; Homeobox protein Nkx-2.5; NK2 transcription factor related locus 5; NK2 transcription factor related, locus 5; NKX 2-5; Nkx-25; tinman paralog

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Gene Aliases: CHNG5; CSX; CSX1; HLHS2; Nkx-2.5; NKX2-5; NKX2.5; NKX2E; NKX4-1; tinman; VSD3

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UniProt ID: (Human) P52952, (Mouse) P42582

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Entrez Gene ID: (Human) 1482, (Mouse) 18091

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription cofactor activity transcriptional activator activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding serum response element binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity histone deacetylase binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter vasculogenesis heart looping heart morphogenesis outflow tract septum morphogenesis outflow tract morphogenesis cardiac conduction system development atrioventricular node development bundle of His development Purkinje myocyte differentiation cardiac ventricle morphogenesis cardiac ventricle formation right ventricular cardiac muscle tissue morphogenesis ventricular trabecula myocardium morphogenesis apoptotic process involved in heart morphogenesis septum secundum development proepicardium development pulmonary myocardium development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development endoderm development heart development adult heart development positive regulation of cell proliferation positive regulation of gene expression negative regulation of cardiac muscle cell apoptotic process positive regulation of transcription via serum response element binding positive regulation of sodium ion transport negative regulation of myotube differentiation hemopoiesis cell differentiation BMP signaling pathway thyroid gland development embryonic heart tube development negative regulation of apoptotic process tongue development sarcomere organization positive regulation of neuron differentiation positive regulation of heart contraction positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter spleen development cardiac muscle tissue development positive regulation of cardioblast differentiation ventricular cardiac myofibril assembly cardiac muscle cell differentiation cardiac muscle tissue morphogenesis atrial cardiac muscle cell development ventricular cardiac muscle cell development regulation of cardiac muscle contraction pharyngeal system development cardiac muscle cell proliferation regulation of cardiac muscle cell proliferation heart contraction cardiac muscle contraction canonical Wnt signaling pathway positive regulation of transcription initiation from RNA polymerase II promoter heart trabecula formation ventricular septum morphogenesis atrial septum morphogenesis atrioventricular node cell development atrioventricular node cell fate commitment embryonic heart tube left/right pattern formation cardiovascular system development negative regulation of canonical Wnt signaling pathway positive regulation of voltage-gated calcium channel activity negative regulation of transcription, DNA-templated regulation of cardiac conduction
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