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  • Nidogen Antibodies

Invitrogen

Nidogen Monoclonal Antibody (302117)

View all (16) Nidogen antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite Nidogen Monoclonal Antibody (302117)

  • Antibody Testing Data (2)
Nidogen Antibody in Immunocytochemistry (ICC/IF)
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Nidogen Antibody in Immunocytochemistry (ICC/IF)
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Nidogen Antibody (MA5-23911) in ICC/IF

Immunocytochemistry analysis of Nidogen in immersion fixed human mesenchymal stem cells differentiated into chondrocytes. Samples were incubated in Nidogen monoclonal antibody (Product # MA5-23911) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow) and counterstained with DAPI (blue). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Nidogen Antibody in Immunocytochemistry (ICC/IF)
Nidogen Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Nidogen Monoclonal Antibody (302117)

Product Details

MA5-23911

Applications
Tested Dilution
Publications

Western Blot (WB)

1 µg/mL
-

Immunohistochemistry (IHC)

3-25 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

3-25 µg/mL
-

Immunocytochemistry (ICC/IF)

3-25 µg/mL
-

ELISA (ELISA)

0.1-0.4 µg/mL (Detection), 2-8 µg/mL (Capture)
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

302117

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Nidogen-1/Entactin Leu29-Lys1114 (Gln1113Arg)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609420

Product Specific Information

In direct ELISAs and Western blots, no cross-reactivity with recombinant human Nidogen-2 is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: enactin; Entactin; NID 1; NID-1; Nidogen (enactin); Nidogen-1; Nidogen-1;

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Gene Aliases: NID; NID1

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UniProt ID: (Human) P14543

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Entrez Gene ID: (Human) 4811

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Function(s)
calcium ion binding collagen binding laminin binding laminin-1 binding proteoglycan binding
Process(es)
cell-matrix adhesion positive regulation of cell-substrate adhesion extracellular matrix disassembly extracellular matrix organization glomerular basement membrane development basement membrane organization
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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