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  • Neutrophil elastase Antibodies

Invitrogen

Neutrophil elastase Monoclonal Antibody (ELA10-101.5)

1 Published Figure
1 Reference
View all (30) Neutrophil elastase antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite Neutrophil elastase Monoclonal Antibody (ELA10-101.5)

  • Published Figures (1)
Neutrophil elastase Antibody in ELISA (ELISA)
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Neutrophil elastase Antibody in ELISA (ELISA)
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FIGURE: 1 / 1

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Neutrophil elastase Antibody (MA1-10608) in ELISA

PLoS pathogens 2016 - Fig 2 Flagellum is the main contributor to NET release triggered by P . aeruginosa . (A) Human PMNs were exposed to WT (PAK or PAO1) and flagellum-deficient strains (PAK flgC and PAO1 fliC ) of P . aeruginosa (10 MOI), and the following readouts were measured: (A) Release of myeloperoxidase (MPO) and human neutrophil elastase (HNE) measured by ELISA. MPO: PAK ( n = 5), PAO1 ( n = 4); HNE: PAK ( n = 11), PAO... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Neutrophil elastase Antibody in ELISA (ELISA)
Neutrophil elastase Monoclonal Antibody (ELA10-101.5)

Product Details

MA1-10608

Applications
Tested Dilution
Publications

ELISA (ELISA)

1-10 µg/mL
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ELA10-101.5

Immunogen

Purified human Neutrophil Elastase.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.4 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1077224

Product Specific Information

MA1-10608 detects Neutrophil elastase from human samples.

MA1-10608 has been successfully used in ELISA and in studies of inhibition and enzyme kinetics of neutrophil elastase.

The MA1-10608 immunogen is purified human Neutrophil Elastase.

Target Information

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Bone marrow serine protease; ELA2A; Elastase; elastase 2, neutrophil; Elastase-2; Elastase2; granulocyte-derived elastase; HLE; Human leukocyte elastase; leukocyte elastase; Medullasin; Neutrophil elastase; PE-1; PMN elastase; polymorphonuclear elastase

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Gene Aliases: ELA2; ELANE; GE; HLE; HNE; NE; PMN-E; SCN1

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UniProt ID: (Human) P08246

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Entrez Gene ID: (Human) 1991

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Function(s)
RNA polymerase II transcription corepressor activity protease binding endopeptidase activity serine-type endopeptidase activity protein binding heparin binding peptidase activity cytokine binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to yeast acute inflammatory response to antigenic stimulus proteolysis cellular calcium ion homeostasis phagocytosis response to UV extracellular matrix disassembly protein catabolic process response to lipopolysaccharide defense response to bacterium positive regulation of MAP kinase activity negative regulation of growth of symbiont in host negative regulation of chemokine biosynthetic process negative regulation of interleukin-8 biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of smooth muscle cell proliferation negative regulation of inflammatory response positive regulation of immune response leukocyte migration negative regulation of chemotaxis neutrophil mediated killing of fungus
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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