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  • Neutrophil elastase Antibodies

Invitrogen

Neutrophil elastase Recombinant Rabbit Monoclonal Antibody (8X4E6)

View all (30) Neutrophil elastase antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite Neutrophil elastase Recombinant Rabbit Monoclonal Antibody (8X4E6)

  • Antibody Testing Data (3)
Neutrophil elastase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Neutrophil elastase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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Neutrophil elastase Antibody (MA5-42901) in IHC (P)

Immunohistochemistry analysis of Neutrophil elastase in paraffin-embedded mouse bone marrow. Samples were incubated with Neutrophil elastase Monoclonal antibody (Product # MA5-42901) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Neutrophil elastase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Neutrophil elastase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Neutrophil elastase Antibody in Western Blot (WB)
Neutrophil elastase Recombinant Rabbit Monoclonal Antibody (8X4E6)

Product Details

MA5-42901

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

8X4E6

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 167-267 of human Neutrophil Elastase (ELANE) (P08246).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2912042

Product Specific Information

Positive test controls include: THP-1, U-937.

Immunogen sequence: SVLQELNVTV VTSLCRRSNV CTLVRGRQAG VCFGDSGSPL VCNGLIHGIA SFVRGGCASG LYPDAFAPVA QFVNWIDSII QRSEDNPCPH PRDPDPASRT H

Target Information

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bone marrow serine protease; ELA2A; Elastase; elastase 2, neutrophil; Elastase-2; Elastase2; granulocyte-derived elastase; HLE; Human leukocyte elastase; Leukocyte elastase; Medullasin; Neutrophil elastase; PE-1; PMN elastase; polymorphonuclear elastase

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Gene Aliases: ELA2; ELANE; F430011M15Rik; GE; HLE; HNE; NE; PMN-E; SCN1

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UniProt ID: (Human) P08246, (Mouse) Q3UP87

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Entrez Gene ID: (Human) 1991, (Mouse) 50701

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Function(s)
RNA polymerase II transcription corepressor activity protease binding endopeptidase activity serine-type endopeptidase activity protein binding heparin binding peptidase activity cytokine binding serine-type peptidase activity hydrolase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to yeast acute inflammatory response to antigenic stimulus proteolysis cellular calcium ion homeostasis phagocytosis response to UV extracellular matrix disassembly protein catabolic process response to lipopolysaccharide defense response to bacterium positive regulation of MAP kinase activity negative regulation of growth of symbiont in host negative regulation of chemokine biosynthetic process negative regulation of interleukin-8 biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of smooth muscle cell proliferation negative regulation of inflammatory response positive regulation of immune response leukocyte migration negative regulation of chemotaxis neutrophil mediated killing of fungus transcription from RNA polymerase II promoter defense response to fungus
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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