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  • Primary Antibodies ›
  • Neutrophil elastase Antibodies

Invitrogen

Neutrophil elastase Polyclonal Antibody

View all (30) Neutrophil elastase antibodies

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Datasheet
Protocols
Questions & Answers

Cite Neutrophil elastase Polyclonal Antibody

  • Antibody Testing Data (5)
Neutrophil elastase Antibody in Immunocytochemistry (ICC/IF)
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Neutrophil elastase Antibody in Immunocytochemistry (ICC/IF)
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Neutrophil elastase Antibody (PA5-115648) in ICC/IF

Immunocytochemistry analysis of Neutrophil elastase in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115648). Secondary staining was applied with mouse anti-beta tubulin (1 hr at 37°, AlexaFluor 594 conjugated goat anti-rabbit IgG (Red), AlexaFluor 488 conjugated goat anti-mouse IgG... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Neutrophil elastase Antibody in Immunocytochemistry (ICC/IF)
Neutrophil elastase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Neutrophil elastase Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Neutrophil elastase Antibody in Western Blot (WB)
Neutrophil elastase Antibody in Western Blot (WB)
Neutrophil elastase Polyclonal Antibody

Product Details

PA5-115648

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ELANE(Accession P08246), corresponding to amino acid residues A231-H267.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2900283

Product Specific Information

Antibody detects endogenous levels of total Neutrophil Elastase.

Target Information

The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). It is present in azurophil granules in the neutrophil cytoplasm. There appear to be two forms of neutrophil elastase, termed IIa and IIb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Bone marrow serine protease; ELA2A; Elastase; elastase 2, neutrophil; Elastase-2; Elastase2; granulocyte-derived elastase; HLE; Human leukocyte elastase; Leukocyte elastase; Medullasin; Neutrophil elastase; PE-1; PMN elastase; polymorphonuclear elastase

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Gene Aliases: ELA2; ELANE; F430011M15Rik; GE; HLE; HNE; NE; PMN-E; SCN1

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UniProt ID: (Human) P08246, (Mouse) Q3UP87

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Entrez Gene ID: (Human) 1991, (Rat) 299606, (Mouse) 50701

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Function(s)
RNA polymerase II transcription corepressor activity protease binding endopeptidase activity serine-type endopeptidase activity protein binding heparin binding peptidase activity cytokine binding molecular_function serine-type peptidase activity hydrolase activity
Process(es)
negative regulation of transcription from RNA polymerase II promoter response to yeast acute inflammatory response to antigenic stimulus proteolysis cellular calcium ion homeostasis phagocytosis response to UV extracellular matrix disassembly protein catabolic process response to lipopolysaccharide defense response to bacterium positive regulation of MAP kinase activity negative regulation of growth of symbiont in host negative regulation of chemokine biosynthetic process negative regulation of interleukin-8 biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of smooth muscle cell proliferation negative regulation of inflammatory response positive regulation of immune response leukocyte migration negative regulation of chemotaxis neutrophil mediated killing of fungus biological_process defense response to fungus transcription from RNA polymerase II promoter
It has to be done as per old AB suggested Products section.
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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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