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Alomone Labs, LTD

Neurotrophin 4 (NT-4) Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers

Cite Neurotrophin 4 (NT-4) Polyclonal Antibody

  • Antibody Testing Data (2)
Neurotrophin 4 (NT-4) Antibody in Immunohistochemistry (IHC)
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Neurotrophin 4 (NT-4) Antibody in Immunohistochemistry (IHC)
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Neurotrophin 4 (NT-4) Antibody (ANT-004-200UL) in IHC

Expression of Neurotrophin 4 in mouse spinal cord - Immunohistochemical staining of mouse spinal cord using Anti-Neurotrophin 4 (NT-4) Antibody (#ANT-004). The spinal ventral horn (VH) was very lightly stained. However, in the perimeterof the white matter (WM) there were NT-4 immunoreactive cells with a small soma (triangles) and some had a stained process (arrows). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Neurotrophin 4 (NT-4) Antibody in Immunohistochemistry (IHC)
Neurotrophin 4 (NT-4) Antibody in Western Blot (WB)
Neurotrophin 4 (NT-4) Polyclonal Antibody

Product Details

ANT-004-200UL

Applications
Tested Dilution
Publications

Western Blot (WB)

1:200
-

Immunohistochemistry (IHC)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

LGEVPAAGGSPL(C), corresponding to amino acid residues 43-52 of mature human NT-4(residues 123-132 of the precursor NT-4), Intracellular
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 5% sucrose, 1% BSA

Contains

0.025% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: neurotrophic factor 4; neurotrophic factor 5; Neurotrophin 4/5; Neurotrophin 5 (neurotrophin 4/5); neurotrophin 5 (neurotrophin 4/5); Neurotrophin-4; Neurotrophin-5; Neutrophic factor 4; Neutrophic factor 5; NT-4; NT-5; NT4/NT5

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Gene Aliases: 2900040K06Rik; AI462899; GLC10; GLC1O; NT-4; NT-4/5; NT-5; NT4; NT4/5; NT4P; NT5; Ntf-5; NTF4; NTF5

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UniProt ID: (Human) P34130, (Rat) P34131, (Mouse) Q80VU4

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Entrez Gene ID: (Human) 4909, (Rat) 25730, (Mouse) 78405

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Function(s)
neurotrophin p75 receptor binding protein binding growth factor activity receptor binding
Process(es)
transmembrane receptor protein tyrosine kinase signaling pathway cell-cell signaling ganglion mother cell fate determination long-term memory sensory organ boundary specification adult locomotory behavior epidermis development mechanoreceptor differentiation negative regulation of neuron apoptotic process regulation of neuron differentiation neuron projection morphogenesis innervation taste bud development regulation of synaptic plasticity negative regulation of cell death
It has to be done as per old AB suggested Products section.
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