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NeoBiotechnologies

Neurofilament (NF-H) (Neuronal Marker) Monoclonal Antibody (NF421)

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite Neurofilament (NF-H) (Neuronal Marker) Monoclonal Antibody (NF421)

  • Antibody Testing Data (6)
Neurofilament (NF-H) (Neuronal Marker) Antibody in Immunocytochemistry (ICC/IF)
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Neurofilament (NF-H) (Neuronal Marker) Antibody in Immunocytochemistry (ICC/IF)
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Neurofilament (NF-H) (Neuronal Marker) Antibody (4744-MSM1-P0) in ICC/IF

Immunofluorescence staining of HEK293 cells using Neurofilament Mouse Monoclonal Antibody (NF421) followed by goat anti-Mouse IgG conjugated to CF488 (green). Membrane stained with Phalloidin (Red). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Neurofilament (NF-H) (Neuronal Marker) Antibody in Immunocytochemistry (ICC/IF)
Neurofilament (NF-H) (Neuronal Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Neurofilament (NF-H) (Neuronal Marker) Antibody in Immunohistochemistry (Paraffin) (IHC (P))
Neurofilament (NF-H) (Neuronal Marker) Antibody in Western Blot (WB)
Neurofilament (NF-H) (Neuronal Marker) Antibody in Flow Cytometry (Flow)
Neurofilament (NF-H) (Neuronal Marker) Antibody in SDS-PAGE (SDS-PAGE)
Neurofilament (NF-H) (Neuronal Marker) Monoclonal Antibody (NF421)

Product Details

4744-MSM1-P0

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunohistochemistry (PFA fixed) (IHC (PFA))

0.25-0.5 µg/mL
-

Immunocytochemistry (ICC/IF)

1-2 µg/mL
-

Flow Cytometry (Flow)

1-2 µg/10^6 cells
-
Product Specifications

Species Reactivity

Chicken, Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

NF421

Immunogen

Recombinant full-length human neurofilament (NEFH) protein
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Wet ice

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 200 kDa neurofilament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC

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Gene Aliases: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH

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UniProt ID: (Human) P12036, (Mouse) P19246

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Entrez Gene ID: (Human) 4744, (Rat) 24587, (Mouse) 380684

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Function(s)
structural molecule activity structural constituent of cytoskeleton microtubule binding toxic substance binding kinesin binding protein kinase binding protein binding, bridging dynein binding protein heterodimerization activity nutrient reservoir activity
Process(es)
microtubule cytoskeleton organization ovarian follicle atresia axonogenesis spinal cord development hippocampus development cerebral cortex development cell projection assembly axon regeneration neurofilament bundle assembly cellular response to oxidative stress response to cocaine peripheral nervous system neuron axonogenesis axon development cellular response to estradiol stimulus regulation of organelle transport along microtubule response to sodium arsenite response to acrylamide brain development embryo development neuron projection development intermediate filament cytoskeleton organization intermediate filament bundle assembly neurofilament cytoskeleton organization
It has to be done as per old AB suggested Products section.
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