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OriGene

NT3 Monoclonal Antibody (OTI5A2), TrueMAB™

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Protocols
Questions & Answers

Cite NT3 Monoclonal Antibody (OTI5A2), TrueMAB™

  • Antibody Testing Data (10)
NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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NT3 Antibody (CF500030) in IHC (P)

Immunohistochemical staining of paraffin-embedded endometrium within the normal limits using anti-NTF3(TA500030) mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NT3 Antibody in Western Blot (WB)
NT3 Antibody in Western Blot (WB)
NT3 Monoclonal Antibody (OTI5A2), TrueMAB™

Product Details

CF500030

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI5A2

Immunogen

Recombinant protein expressed in E.coli corresponding to amino acids 139-257 of human NT3
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: HDNF; Nerve growth factor 2; Neurotrophic factor; Neurotrophin-3; NGF-2; NT-3

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Gene Aliases: HDNF; NGF-2; NGF2; NT-3; NT3; NTF3

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UniProt ID: (Human) P20783

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Entrez Gene ID: (Human) 4908

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Function(s)
receptor binding neurotrophin p75 receptor binding protein binding growth factor activity chemoattractant activity nerve growth factor binding
Process(es)
activation of MAPK activity positive regulation of receptor internalization signal transduction transmembrane receptor protein tyrosine kinase signaling pathway cell-cell signaling nervous system development positive regulation of cell proliferation positive regulation of cell migration activation of protein kinase B activity positive regulation of peptidyl-serine phosphorylation myelination regulation of apoptotic process negative regulation of neuron apoptotic process regulation of neuron differentiation positive regulation of glial cell differentiation neuron projection morphogenesis positive regulation of peptidyl-tyrosine phosphorylation negative regulation of peptidyl-tyrosine phosphorylation modulation of synaptic transmission positive chemotaxis induction of positive chemotaxis activation of GTPase activity positive regulation of actin cytoskeleton reorganization
It has to be done as per old AB suggested Products section.
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