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  • NRF1 Antibodies

Invitrogen

NRF1 Polyclonal Antibody

View all (26) NRF1 antibodies

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Cite NRF1 Polyclonal Antibody

  • Antibody Testing Data (21)
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
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NRF1 Antibody (PA5-95573) in IHC (P)

Immunohistochemistry analysis of NRF1 in paraffin-embedded human gastric cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with NRF1 polyclonal antibody (Product # PA5-95573) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Complex a... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
NRF1 Antibody in Western Blot (WB)
NRF1 Antibody in Flow Cytometry (Flow)
NRF1 Antibody in Flow Cytometry (Flow)
NRF1 Polyclonal Antibody

Product Details

PA5-95573

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human NRF1 recombinant protein (Position: D246-Q503).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice

RRID

AB_2807375

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human K562 whole cell, human MDA-MB-231 whole cell. IHC: human placenta tissue, human ovary cancer tissue, mouse intestine tissue, mouse kidney tissue, mouse spleen tissue, rat heart tissue, rat kidney tissue, human appendicitis tissue, human gastric cancer tissue, human tonsil tissue, mouse cardiac muscle tissue, mouse liver tissue, rat brain tissue, rat intestine tissue, rat liver tissue, rat lung tissue, rat skeletal muscle tissue, rat spleen tissue. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Alpha palindromic-binding protein; Alpha-pal; EWG; NRF-1; Nuclear respiratory factor 1; nuclear respiratory factor-1

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Gene Aliases: ALPHA-PAL; C87038; D6Ertd415e; Nfe2l1_retired; NRF1

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UniProt ID: (Human) Q16656, (Mouse) Q9WU00

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Entrez Gene ID: (Human) 4899, (Rat) 312195, (Mouse) 18181

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding protein homodimerization activity molecular_function DNA binding transcription factor activity, sequence-specific DNA binding
Process(es)
response to hypoxia transcription, DNA-templated mitochondrion organization organ regeneration response to estradiol response to lipopolysaccharide positive regulation of transcription from RNA polymerase II promoter response to folic acid response to electrical stimulus regulation of transcription, DNA-templated transcription from RNA polymerase II promoter generation of precursor metabolites and energy regulation of transcription from RNA polymerase II promoter
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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