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  • Primary Antibodies ›
  • NOX5 Antibodies

Invitrogen

NOX5 Polyclonal Antibody

View all (10) NOX5 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Cite NOX5 Polyclonal Antibody

  • Antibody Testing Data (3)
NOX5 Antibody in Immunocytochemistry (ICC/IF)
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NOX5 Antibody in Immunocytochemistry (ICC/IF)
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NOX5 Antibody (PA5-95572) in ICC/IF

Immunocytochemistry analysis of NOX5 using anti-NOX5 antibody (Product # PA5-95572) . NOX5 was detected in a section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-NOX5 antibody (Product # PA5-95572) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visua... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NOX5 Antibody in Immunocytochemistry (ICC/IF)
NOX5 Antibody in Western Blot (WB)
NOX5 Antibody in Flow Cytometry (Flow)
NOX5 Polyclonal Antibody

Product Details

PA5-95572

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunocytochemistry (ICC/IF)

2 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-

ELISA (ELISA)

0.1-0.5 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human NOX5 recombinant protein (Position: E13-F197).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2807374

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human placenta tissue, human SGC-7901 whole cell, human Jurkat whole cell, human THP-1 whole cell. ICC/IF: Hela cell. Flow: PC-3.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

This gene is predominantly expressed in the testis and lymphocyte-rich areas of spleen and lymph nodes. It encodes a calcium-dependen NADPH oxidase that generates superoxide, and functions as a calcium-dependent proton channel that may regulate redox-dependent processes in lymphocytes and spermatozoa. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: NADPH oxidase 5; NOX5zeta

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Gene Aliases: NOX5

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UniProt ID: (Human) Q96PH1

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Entrez Gene ID: (Human) 79400

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Function(s)
calcium ion binding hydrogen ion channel activity superoxide-generating NADPH oxidase activity heme binding flavin adenine dinucleotide binding NADP binding
Process(es)
response to reactive oxygen species cytokinesis angiogenesis endothelial cell proliferation apoptotic process cell proliferation regulation of proton transport proton transport superoxide anion generation regulation of fusion of sperm to egg plasma membrane cytokine secretion oxidation-reduction process
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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