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NOS3 Monoclonal Antibody (1D12)

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers
NOS3 Monoclonal Antibody (1D12)

Product Details

H00004846-M04

Applications
Tested Dilution
Publications

Western Blot (WB)

1-5 µg/mL
-

ELISA (ELISA)

3 ng/mL
-

in situ PLA (PLA)

1:1200
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1D12

Immunogen

NOS3 (AAH63294.1, 61 a.a. approximately 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: QPPEGPKFPR VKNWEVGSIT YDTLSAQAQQ DGPCTPRRCL GSLVFPRKLQ GRPSPGPPAP EQLLSQARDF INQYYSSIKR SGSQAHEQRL QEVEAEVAAT

Target Information

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) and endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cNOS; Constitutive NOS; e-NOS; EC-NOS; Endothelial NOS; Nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3 transcript variant eNOS-delta20; nitric oxide synthase 3 transcript variant eNOS-delta20-21; nitric oxide synthase 3 transcript variant eNOS-delta21; Nitric oxide synthase, endothelial; NOS type III; NOSIII; OTTHUMP00000213183

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Gene Aliases: ECNOS; eNOS; NOS3

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UniProt ID: (Human) P29474

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Entrez Gene ID: (Human) 4846

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Function(s)
actin monomer binding nitric-oxide synthase activity iron ion binding protein binding calmodulin binding FMN binding heme binding tetrahydrobiopterin binding arginine binding cadmium ion binding flavin adenine dinucleotide binding NADP binding scaffold protein binding
Process(es)
angiogenesis ovulation from ovarian follicle in utero embryonic development blood vessel remodeling regulation of sodium ion transport regulation of the force of heart contraction by chemical signal regulation of systemic arterial blood pressure by endothelin arginine catabolic process nitric oxide biosynthetic process mitochondrion organization nitric oxide mediated signal transduction regulation of blood pressure negative regulation of cell proliferation response to heat negative regulation of platelet activation negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals lung development positive regulation of guanylate cyclase activity lipopolysaccharide-mediated signaling pathway response to fluid shear stress negative regulation of potassium ion transport endothelial cell migration cell redox homeostasis positive regulation of angiogenesis negative regulation of blood pressure positive regulation of vasodilation regulation of blood vessel size regulation of nitric-oxide synthase activity negative regulation of hydrolase activity negative regulation of calcium ion transport oxidation-reduction process negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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