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  • Primary Antibodies ›
  • NMT1 Antibodies

Bethyl Laboratories

NMT1 Polyclonal Antibody

View all (9) NMT1 antibodies

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Datasheet
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Datasheet
Protocols
Questions & Answers

Cite NMT1 Polyclonal Antibody

  • Antibody Testing Data (2)
NMT1 Antibody in Western Blot (WB)
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NMT1 Antibody in Western Blot (WB)
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NMT1 Antibody (A304-253A) in WB

Detection of human and mouse NMT1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NMT1 antibody (Product # A304-253A) (lot A304-253A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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NMT1 Antibody in Western Blot (WB)
NMT1 Antibody in Immunoprecipitation (IP)
NMT1 Polyclonal Antibody

Product Details

A304-253A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000-1:10,000
-

Immunoprecipitation (IP)

2-10 µg/mg lysate
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 50 to 100 of human N-Myristoyltransferase 1.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

phosphate/tris citrate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine and Orangutan

Target Information

NMT1 is involved with Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1 MIM 139311).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alternative, short form NMT-S; Glycylpeptide N-tetradecanoyltransferase 1; HsNMT1; long form, NMT-L; Myristoyl-CoA:protein N-myristoyltransferase 1; NMT 1; Peptide N-myristoyltransferase 1; Protein-lysine myristoyltransferase NMT1; type I N-myristoyltransferase

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Gene Aliases: AW536594; NMT; NMT1

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UniProt ID: (Human) P30419, (Mouse) O70310

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Entrez Gene ID: (Human) 4836, (Mouse) 18107

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Function(s)
glycylpeptide N-tetradecanoyltransferase activity myristoyltransferase activity transferase activity transferase activity, transferring acyl groups
Process(es)
in utero embryonic development N-terminal protein myristoylation N-terminal peptidyl-glycine N-myristoylation regulation of rhodopsin mediated signaling pathway positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
It has to be done as per old AB suggested Products section.
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